PMID: 9164987May 1, 1997Paper

Lactase phlorizin hydrolase synthesis is decreased in protein-malnourished pigs

The Journal of Nutrition
M A DudleyP J Reeds

Abstract

We have examined the effect of protein malnutrition on brush border (BB) lactase phlorizin hydrolase (LPH) synthesis in young pigs. Two groups of four 3-wk-old pigs were fed diets containing either 19 g soy protein, 63 g carbohydrate and 5 g fat per 100 g diet (a protein-sufficient diet) or 3 g soy protein, 85 g carbohydrate and 5 g fat per 100 g diet (a protein-deficient diet). After 8 wk of consuming the diets, pigs were infused intravenously with 2H3-leucine for 8 h, then killed. The jejunum was collected for measurement of lactase activity, LPH mRNA abundance and the rate of LPH post-translational synthesis. Lactase activities did not differ between groups (mean 8.1 +/- 1.2 micromol x min(-1) x g mucosa(-1)). LPH mRNA abundance relative to elongation factor-1alpha mRNA (the constitutive/reference mRNA) was significantly (P < 0.05) higher in well-nourished pigs (0.36 +/- 0.03%) than in protein-malnourished pigs (0.21 +/- 0.02%). The rate constants of BB LPH post-translational synthesis were also significantly higher in the well-nourished (103 +/- 9% x d(-1)) than in the protein-malnourished pigs (66 +/- 8% x d(-1)). Further, the absolute synthesis rate of BB LPH, a measure of the amount of enzyme synthesized per gram of tiss...Continue Reading

References

Nov 1, 1977·Clinical Science and Molecular Medicine·M H GoldenD Picou
Oct 1, 1992·Journal of Pediatric Gastroenterology and Nutrition·M A DudleyB L Nichols
Mar 1, 1992·The Journal of Nutrition·M A DudleyP J Reeds
Feb 1, 1992·Journal of Pediatric Gastroenterology and Nutrition·R J ShulmanS J Henning
Jul 1, 1984·The Biochemical Journal·E M DanielsenH Sjöström
Jul 18, 1984·Biochemical and Biophysical Research Communications·E M DanielsenH Sjöström
Jun 15, 1982·The Biochemical Journal·M A McNurlanP J Garlick
Jul 1, 1996·Journal of Pediatric Gastroenterology and Nutrition·R K Buddington, C Malo

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Citations

Feb 12, 1998·The American Journal of Physiology·M A DudleyP J Reeds
Mar 20, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M A DudleyP J Reeds
Dec 1, 2009·Archives of Animal Nutrition·Florence Omobola OpapejuCharles Martin Nyachoti
Mar 30, 2005·The Journal of Nutrition·Maaike W SchaartJohannes B van Goudoever
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J B van GoudoeverP J Reeds

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