Lactate as substrate for mitochondrial respiration in alveolar epithelial type II cells

American Journal of Physiology. Lung Cellular and Molecular Physiology
Robyn G LottesJohn E Baatz

Abstract

Because of the many energy-demanding functions they perform and their physical location in the lung, alveolar epithelial type II (ATII) cells have a rapid cellular metabolism and the potential to influence substrate availability and bioenergetics both locally in the lung and throughout the body. A thorough understanding of ATII cell metabolic function in the healthy lung is necessary for determining how metabolic changes may contribute to pulmonary disease pathogenesis; however, lung metabolism is poorly understood at the cellular level. Here, we examine lactate utilization by primary ATII cells and the ATII model cell line, MLE-15, and link lactate consumption directly to mitochondrial ATP generation. ATII cells cultured in lactate undergo mitochondrial respiration at near-maximal levels, two times the rates of those grown in glucose, and oxygen consumption under these conditions is directly linked to mitochondrial ATP generation. When both lactate and glucose are available as metabolic substrate, the presence of lactate alters glucose metabolism in ATII to favor reduced glycolytic function in a dose-dependent manner, suggesting that lactate is used in addition to glucose when both substrates are available. Lactate use by ATII...Continue Reading

References

Jan 1, 1986·Experimental Lung Research·C E PattersonR A Rhoades
May 1, 1984·The American Journal of Physiology·A B Fisher, C Dodia
Jan 1, 1982·Basic Research in Cardiology·A J Drake
Apr 1, 1996·The Journal of Nutrition·R E FoxJ T Tildon
Mar 17, 2001·Microbes and Infection·A M LeVine, J A Whitsett
May 1, 2007·Sports Medicine·George A Brooks
Apr 2, 2008·Medicine and Science in Sports and Exercise·Takeshi Hashimoto, George A Brooks
Sep 2, 2008·Proceedings of the American Thoracic Society·Wellington V Cardoso, Jeffrey A Whitsett
Jun 27, 2009·Analytical Chemistry·Akos A GerencserMartin D Brand
Oct 7, 2009·The Journal of Physiology·George A Brooks
Oct 31, 2009·Pflügers Archiv : European journal of physiology·Fátima Pérez de HerediaPaul Trayhurn
Jan 8, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zissis C ChroneosVirginia L Shepherd
Oct 16, 2010·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Tobias BenselDieter Worlitzsch
Oct 22, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fawzi BoumezbeurDouglas L Rothman
Jun 17, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew L JohnsonGeorge A Brooks
Oct 28, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew L JohnsonGeorge A Brooks
Dec 14, 2011·IUBMB Life·Andrew P Halestrap, Marieangela C Wilson
Aug 28, 2012·American Journal of Respiratory and Critical Care Medicine·Robert Matthew KottmannPatricia J Sime
Aug 31, 2013·The International Journal of Biochemistry & Cell Biology·Loïc GuillotAnnick Clement
Dec 21, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Sara GranjaFátima Baltazar
Apr 1, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robyn G LottesJohn E Baatz

❮ Previous
Next ❯

Citations

Nov 22, 2017·Annals of the American Thoracic Society·Charalambos MichaeloudesIan M Adcock
Oct 16, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y S Prakash
Dec 25, 2019·Cellular and Molecular Life Sciences : CMLS·Justin B Collier, Rick G Schnellmann
Oct 6, 2020·Oxidative Medicine and Cellular Longevity·Patrick C BradshawWilliam M Curtis
Jul 6, 2018·Journal of Neuroinflammation·Elizaveta B BoitsovaAlla B Salmina
Sep 13, 2020·The Journal of Biological Chemistry·Jennifer L Larson-CaseyA Brent Carter
Dec 24, 2018·Ecotoxicology and Environmental Safety·Xia NingNan Sang
Apr 8, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yael-Natalie H EscobarIlona Jaspers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.