Lactate metabolism in mouse brain astrocytes studied by [13C]NMR spectroscopy

Neuroreport
P M AlvesUrsula Sonnewald

Abstract

Astrocytes possess at least two pathways for pyruvate and thus lactate formation involving precursors derived from mitochondria. The present results suggest that malic enzyme is the preferred route for this process. Although overall lactate release appeared to be independent of extracellular lactate concentration, the incorporation of mitochondrial precursors was decreased by starvation, which is known to deplete astrocyte glycogen stores. Using 1-[13C]glucose in the presence of 10 mM lactate led to label incorporation into extracellular lactate which increased from 3.4 +/- 0.4 to 6.5 +/- 0.5% during 4 and 6 h incubation periods, respectively. Lactate production from glycolysis proceeded virtually unaffected by the extracellular lactate concentration. The total amount of lactate in the medium decreased, however, demonstrating that lactate was used as a substrate.

Citations

Dec 17, 2004·Journal of Neuroscience Research·Sónia Sá SantosPaula M Alves
Dec 2, 2004·Journal of Neuroscience Research·José M Medina, Arantxa Tabernero
Jun 15, 2001·ILAR Journal·J C Chatham, S J Blackband
Mar 20, 2003·Journal of Neuroscience Research·In-Young ChoiRolf Gruetter
May 23, 2014·The Journal of Biological Chemistry·Sanju LamaGarnette R Sutherland
Jun 23, 2020·Developmental Neuroscience·Alkisti MikrogeorgiouDonna M Ferriero

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