PMID: 9526973Apr 4, 1998Paper

Lactate production from glucose and response to insulin in perifused adipocytes from mesenteric and epididymal regions of lean and obese rats

Obesity Research
J L King, M DiGirolamo

Abstract

Lactate, an important metabolic substrate for peripheral tissues and the liver, is released in significant amounts from adipose tissue. Using a perifusion system, we measured lactate production from glucose and response to insulin in isolated mesenteric and epididymal adipocytes removed from fed or fasted male Wistar rats at two stages of growth and development: (a) lean rats (7 weeks to 9 weeks old, weighing approximately 250 g), and (b) fatter rats (6 months to 8 months old, weighing approximately 550 g). The results show that lactate production in perifused adipocytes is regulated by the prior nutritional state of the animals, by the adipose tissue region, and by the presence of insulin in the perifusate. In fat cells from lean rats, basal lactate production was significantly higher (p<0.05) in mesenteric cells when compared with epididymal cells, both in the fed state (7.8 nmo/10(7) fat cells per minute vs. 2.9 nmol/10(7) fat cells per minute) and after 2 days of fasting (13.6 nmol vs. 3.5 nmol). When the response to 1 mU/mL insulin was studied, however, the relative increase in lactate production produced by insulin was greater in the epididymal cells than in the mesenteric cells, in both the fed (194% vs. 91% over basal, ...Continue Reading

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Citations

Sep 7, 2001·Diabetes/metabolism Research and Reviews·J Radziuk, S Pye
Mar 6, 2004·Journal of Physiology and Biochemistry·C BairrasC Atgié
Aug 4, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mary C SugdenMark J Holness
Sep 15, 2006·Journal of Animal Science·C H ChenS T Ding
May 22, 2019·Obesity·José C E SerranoManuel Portero-Otín

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