Lactate Production Precedes Inflammatory Cell Recruitment in Arthritic Ankles: an Imaging Study.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Marie-Aline NeveuAndreas M Schmid

Abstract

Inflammation is involved in many disease processes. However, accurate imaging tools permitting diagnosis and characterization of inflammation are still missing. As inflamed tissues exhibit a high rate of glycolysis, pyruvate metabolism may offer a unique approach to follow the inflammatory response and disease progression. Therefore, the aim of the study was to follow metabolic changes and recruitment of inflammatory cells after onset of inflammation in arthritic ankles using hyperpolarized 1-13C-pyruvate magnetic resonance spectroscopy (MRS) and 19F magnetic resonance imaging (MRI), respectively. Experimental rheumatoid arthritis (RA) was induced by intraperitoneal injection of glucose-6-phosphate-isomerase-specific antibodies (GPI) containing serum. To monitor pyruvate metabolism, the transformation of hyperpolarized 1-13C-pyruvate into hyperpolarized 1-13C-lactate was followed using MRS. To track phagocytic immune cell homing, we intravenously injected a perfluorocarbon emulsion 48 h before imaging. The animals were scanned at days 1, 3, or 6 after GPI-serum injection to examine the different stages of arthritic inflammation. Finally, to confirm the pyruvate metabolic activity and the link to inflammatory cell recruitment, w...Continue Reading

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Citations

Dec 9, 2020·NMR in Biomedicine·Stephanie AndersonDamian J Tyler
May 18, 2021·Frontiers in Immunology·Sally A ClaytonAndrew R Clark

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Methods Mentioned

BETA
dynamic light scattering

Software Mentioned

Prism
jMRUI
PMOD
Inveon Research Workplace
Paravision
GraphPad

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