PMID: 6159793Nov 1, 1980Paper

Lactate-sodium cotransport in rat renal brush border membranes

The American Journal of Physiology
M Barac-NietoR Kinne

Abstract

Brush border membrane vesicles were obtained from rat kidney cortex through a calcium precipitation method and their transport properties for lactate were studied by a rapid-filtration technique. Transient concentrative uptake of L-lactate was observed in the presence of inwardly directed NaCl gradient, but not in the presence of a KCl, LiCl, RbCl, CsCl, or choline chloride gradient. The sodium-dependent L-lactate uptake was saturable and was inhibited by D-lactate. The activation curve with sodium was hyperbolic. Maneuvers that render the inside of the vesicle more negative stimulated sodium-dependent L-lactate uptake, suggesting an electrogenic transfer of L-lactate and sodium. An L-lactate gradient also accelerates the sodium movement across the brush border membrane. Studies on the pH dependency of L-lactate transport and on the effect of L-lactate on proton movements across the brush border membrane indicate that there is little contribution of nonionic diffusion and/or of lactate-H+ cotransport to the transfer of L-lactate across the renal brush border membrane. In summary, sodium-lactate cotransport is the major mechanism for L-lactate transfer across the renal brush border membrane.

Citations

May 9, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M ToscoA Faelli
Nov 26, 2014·Annual Review of Physiology·Asim Mandal, David B Mount
Jul 15, 1980·The Journal of Membrane Biology·H Murer, R Kinne
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Masako InuyamaYasufumi Sawada
Jul 28, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Van Pham Thi Ha ToMatthew E Loewen
Mar 28, 1985·Biochimica Et Biophysica Acta·K E Jørgensen, M I Sheikh
Feb 1, 1982·The American Journal of Medicine·M G Cogan

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