Lactate transport by rainbow trout white muscle: kinetic characteristics and sensitivity to inhibitors

The American Journal of Physiology
Y WangC M Wood

Abstract

This study used an isolated-perfused tail-trunk preparation of rainbow trout to examine the uptake and release of lactate (Lac) and metabolic protons (delta H+M) in resting and exercised fish white muscle. In exercised muscle, L(+)-Lac efflux was inhibited (approximately 40%) by 5 mM alpha-cyano-4-hydroxycinnamate (CIN), but not by 0.5 mM 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) or 0.1 mM amiloride. These results suggest that Lac release occurs through a Lac(-)-H- symport and the free diffusion of lactic acid (HLac) or Lac-, but not via the Lac-/HCO3(-)-Cl- antiporter. Lac efflux was accompanied by delta H+m influx in all treatments, and increased delta H+m influx occurred after SITS treatment. In resting muscle, Lac uptake rates were greater than Lac efflux rates in the postexercise preparation. L-Lac influx exhibited partial saturation kinetics, whereas D(-)-Lac influx was linearly related to its extracellular concentration (0-32 mM). At 16 mM extracellular L-Lac, with a negligible transmembrane L-HLac gradient and an outwardly directed not driving force on L-Lac-, CIN, and SITS reduced net L-Lac uptake by 75 and 45%, respectively. At 16 mM extracellular concentration, D-Lac influx was 64% of the net L-...Continue Reading

Citations

Aug 15, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Teye OmlinJean-Michel Weber
Nov 16, 2002·Journal of Applied Physiology·Ilka Pinz, Hans-O Pörtner
Jan 23, 2016·The Journal of Experimental Biology·Jean-Michel WeberTeye Omlin
Mar 29, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K R PatonI C Potter
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Mar 29, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Teye Omlin, Jean-Michel Weber
Dec 28, 2002·The Journal of Experimental Biology·Rainie L Sharpe, C Louise Milligan
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Aug 28, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Adam K Ngan, Yuxiang S Wang

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