Lactation Affects Isolated Mitochondria and Its Fatty Acid Composition but Has No Effect on Tissue Protein Oxidation, Lipid Peroxidation or DNA-Damage in Laboratory Mice

Antioxidants
Teresa G ValencakAlois Strasser

Abstract

Linking peak energy metabolism to lifespan and aging remains a major question especially when focusing on lactation in females. We studied, if and how lactation affects in vitro mitochondrial oxygen consumption and mitochondrial fatty acid composition. In addition, we assessed DNA damage, lipid peroxidation and protein carbonyls to extrapolate on oxidative stress in mothers. As model system we used C57BL/6NCrl mice and exposed lactating females to two ambient temperatures (15 °C and 22 °C) while they nursed their offspring until weaning. We found that state II and state IV respiration rates of liver mitochondria were significantly higher in the lactating animals than in non-lactating mice. Fatty acid composition of isolated liver and heart mitochondria differed between lactating and non-lactating mice with higher n-6, and lower n-3 polyunsaturated fatty acids in the lactating females. Surprisingly, lactation did not affect protein carbonyls, lipid peroxidation and DNA damage, nor did moderate cold exposure of 15 °C. We conclude that lactation increases rates of mitochondrial uncoupling and alters mitochondrial fatty acid composition thus supporting the "uncoupling to survive" hypothesis. Regarding oxidative stress, we found no ...Continue Reading

References

Sep 1, 1999·The International Journal of Biochemistry & Cell Biology·J M FaganI Sohar
Oct 29, 2000·Experimental Gerontology·M D Brand
Jul 3, 2002·Experimental Biology and Medicine·Richard A MillerSteven N Austad
Mar 7, 2003·Mutagenesis·Stig Johan Wiklund, Eva Agurell
Jan 29, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea M VincentEva L Feldman
Apr 20, 2006·Mechanisms of Ageing and Development·A J HulbertRochelle Buffenstein
Dec 13, 2006·Aging Cell·Teresa G Valencak, Thomas Ruf
Dec 30, 2008·The Journal of Experimental Biology·T G ValencakT Ruf
May 7, 2010·The Journal of Animal Ecology·John R Speakman, Elzbieta Król
Oct 12, 2010·Proceedings. Biological Sciences·Michael GarrattJane L Hurst
Nov 26, 2011·The Journal of Experimental Biology·Elzbieta KrólJohn R Speakman
Apr 27, 2013·The Journal of Experimental Biology·Nicolas PichaudRobert C Brooks
May 4, 2013·Age·Teresa G Valencak, Thomas Ruf
Nov 29, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·John R Speakman, Michael Garratt
Jan 18, 2014·The Journal of Experimental Biology·Paweł BrzękMarek Konarzewski
Mar 15, 2015·Biological Reviews of the Cambridge Philosophical Society·Jonathan D BlountMichael A Cant

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

lme4
CometScore
Turbochrom

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