Lactation failure in Src knockout mice is due to impaired secretory activation.

BMC Developmental Biology
Harriet WatkinSteven M Anderson

Abstract

Mammary gland development culminates in lactation and is orchestrated by numerous stimuli and signaling pathways. The Src family of nonreceptor tyrosine kinases plays a pivotal role in cell signaling. In order to determine if Src plays a role in mammary gland development we have examined mammary gland development and function during pregnancy and lactation in mice in which expression of Src has been eliminated. We have characterized a lactation defect in the Src-/- mice which results in the death of over 80% of the litters nursed by Src-/- dams. Mammary gland development during pregnancy appears normal in these mice; however secretory activation does not seem to occur. Serum prolactin levels are normal in Src-/- mice compared to wildtype controls. Expression of the prolactin receptor at both the RNA and protein level was decreased in Src-/- mice following the transition from pregnancy to lactation, as was phosphorylation of STAT5 and expression of milk protein genes. These results suggest that secretory activation, which occurs following parturition, does not occur completely in Src-/- mice. Failed secretory activation results in precocious involution in the mammary glands of Src-/- even when pups were suckling. Involution was ...Continue Reading

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Citations

Sep 8, 2010·Oncogene·L HebbardR G Oshima
Nov 11, 2010·Journal of Cell Communication and Signaling·Bethanie Morrison, Mary Lou Cutler
Dec 30, 2014·Fundamental & Clinical Pharmacology·Amit KumarNirmal Singh
Nov 7, 2009·Cellular Signalling·José Manuel García-MartínezJorge Martín-Pérez
Mar 18, 2015·Nature Reviews. Endocrinology·Valérie BernardNadine Binart
May 10, 2019·Genome Génome / Conseil National De Recherches Canada·Periyasamy VijayakumarRamalingam Suresh
Nov 6, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nicholas H McCormickShannon L Kelleher
Feb 9, 2018·PLoS Genetics·Rui ZhangGaoxiang Ge
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Oct 21, 2017·Seminars in Cell & Developmental Biology·Dengbao YangYihong Wan
Nov 15, 2020·Seminars in Cell & Developmental Biology·Oona Paavolainen, Emilia Peuhu

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Methods Mentioned

BETA
immunoprecipitation
GTPases
PCR
protein assay
Assay

Software Mentioned

SlideBook
TaqMan Primer [UNK] software

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