Lactation status influences expression of CCAAT/enhancer binding protein isoform mRNA in the mouse mammary gland

Journal of Cellular Physiology
A P Gigliotti, J DeWille

Abstract

The CCAAT/enhancer binding proteins (C/EBPs) are a highly conserved family of DNA binding proteins implicated in the transcriptional control of genes involved in cell growth and differentiation in a variety of tissues. The expression of C/EBP-alpha, beta, and delta mRNA in the normal mouse mammary gland was investigated during pregnancy, lactation, and involution via Northern blotting and in situ hybridization. Mammary gland C/EBP-alpha mRNA is detectable at low levels during pregnancy and postlactational involution. C/EBP-beta mRNA levels are elevated during pregnancy, decline slightly in midlactation, and are induced within 48 hours of the onset of involution. C/EBP-delta mRNA content is low throughout pregnancy and lactation, but increases dramatically (>100-fold) within 12 hours after the onset of postlactational involution. In situ hybridization demonstrates that mammary epithelial cells are responsible for the expression of C/EBP-delta mRNA during involution. In contrast to mammary gland, C/EBP-alpha is the predominate isoform expressed in liver with relatively low expression of C/EBP-beta and C/EBP-delta mRNA. Liver C/EBP isoform mRNA levels are unaffected by lactation status. These results demonstrate the tissue-specifi...Continue Reading

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Citations

Jan 29, 2000·Journal of Cellular Biochemistry·N CellaN E Hynes
Apr 9, 2008·Apoptosis : an International Journal on Programmed Cell Death·Md Monowar AzizYoshikazu Kinoshita
Feb 27, 2007·Journal of Mammary Gland Biology and Neoplasia·Kate D SutherlandJane E Visvader
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