Lacto-N-fucopentaose III (Lewis x), a target of the antibody response in mice vaccinated with irradiated cercariae of Schistosoma mansoni.

Infection and Immunity
D RichterD A Harn

Abstract

Carbohydrates on soluble egg antigens are major epitopes for the antibody responses of patients and mice infected with Schistosoma mansoni. Recently, protective sera of mice vaccinated with irradiated cercariae were shown to recognize carbohydrate epitopes on schistosomal glutathione S-transferase. The present study demonstrates that carbohydrate epitopes are major targets of sera from C57BL/6J and CBA/J mice vaccinated with 15- or 50-kilorad-irradiated cercariae of S. mansoni. Antibody titers to carbohydrate epitopes increased with the number of vaccinations and were considerably higher in C57BL/6J mice than in CBA/J mice. The specificity of this anticarbohydrate response was determined by measuring antibody binding to defined oligosaccharide residues known to be present on the parasite. A predominant target of the humoral anticarbohydrate response of vaccinated mice was lacto-N-fucopentaose III, a molecule relevant for cell trafficking. We observed no binding to its nonfucosylated homolog, lacto-N-neotetraose, or to oligosaccharides present on keyhole limpet hemocyanin. The strongest antibody response to lacto-N-fucopentaose III was observed for C57BL/6J and CBA/J mice repeatedly vaccinated with 15-kilorad-irradiated cercaria...Continue Reading

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Citations

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Dec 12, 2012·Virulence·Rhiannon R White, Katerina Artavanis-Tsakonas
Sep 24, 2020·Scientific Reports·Ralph N D LuetscherRichard D Cummings
Dec 18, 2003·Cellular Microbiology·Paul G Thomas, Donald A Harn
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May 26, 2004·Archives of Biochemistry and Biophysics·A Kwame NyameRichard D Cummings
Jul 10, 2004·Structure·Anne-Marie M van RoonJan Pieter Abrahams

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