Lactobacilli binding human A-antigen expressed in intestinal mucosa

Research in Microbiology
H UchidaT Saito

Abstract

Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.

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Citations

Jun 18, 2009·Journal of Molecular Evolution·Joana AbrantesJacques Le Pendu
Jun 9, 2009·Bioscience, Biotechnology, and Biochemistry·Kazumasa YasuiTohru Suzuki
Aug 2, 2011·Applied and Environmental Microbiology·Keren Buhnik-RosenblauYechezkel Kashi
Jun 8, 2012·BMC Microbiology·Harri MäkivuokkoJaana Mättö
May 17, 2013·Applied Microbiology and Biotechnology·Ulla Hynönen, Airi Palva
Jun 20, 2013·Animal Biotechnology·Kyungtae KimChankyu Park
Nov 18, 2011·Trends in Microbiology·Nathalie Juge
Jul 19, 2011·Journal of Bioscience and Bioengineering·Nobuhisa AshidaNaoyuki Yamamoto
Nov 26, 2013·Journal of Crohn's & Colitis·Diego ForniManuela Sironi
Oct 1, 2013·Veterinary Immunology and Immunopathology·Tanja LähteinenAiri Palva
Dec 8, 2006·Bioscience, Biotechnology, and Biochemistry·Hideaki UchidaTadao Saito
Mar 4, 2014·PloS One·Antonio Rubio-del-CampoJesús Rodríguez-Díaz
Aug 9, 2016·Progress in Biophysics and Molecular Biology·Chaohua ZhuMing Sun
Sep 30, 2016·Microorganisms·Keita NishiyamaTakao Mukai
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Nov 22, 2016·Bioscience of Microbiota, Food and Health·Hideki KinoshitaTadao Saito

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