Lactobacillus acidophilus Attenuates Salmonella -Induced Stress of Epithelial Cells by Modulating Tight-Junction Genes and Cytokine Responses

Frontiers in Microbiology
Alexia LépinePaul de Vos

Abstract

Scope: Salmonellosis is a prevalent food-borne illness that causes diarrhea in over 130 million humans yearly and can lead to death. There is an urgent need to find alternatives to antibiotics as many salmonellae are now multidrug resistant. As such, specific beneficial bacteria and dietary fibers can be an alternative as they may prevent Salmonella Typhimurium (STM) infection and spreading by strengthening intestinal barrier function. Methods and Results: We tested whether immune active long-chain inulin-type fructans and/or L. acidophilus W37, L. brevis W63, and L. casei W56 can strengthen barrier integrity of intestinal Caco-2 cells in the presence and absence of a STM. Effects of the ingredients on intestinal barrier function were first evaluated by quantifying trans-epithelial electric resistance (TEER) and regulation of gene expression by microarray. Only L. acidophilus had effects on TEER and modulated a group of 26 genes related to tight-junctions. Inulin-type fructans, L. brevis W63 and L. casei W56 regulated other genes, unrelated to tight-junctions. L. acidophilus also had unique effects on a group of six genes regulating epithelial phenotype toward follicle-associated epithelium. L. acidophilus W37 was therefore sel...Continue Reading

References

Feb 13, 2003·Nucleic Acids Research·Rafael A IrizarryTerence P Speed
Jun 19, 2004·Digestion·Massimo MontaltoGiovanni Gasbarrini
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Feb 24, 2006·Nature Reviews. Immunology·Marian R Neutra, Pamela A Kozlowski
Feb 15, 2007·Travel Medicine and Infectious Disease·Lynne V McFarland
Dec 9, 2008·Biochimica Et Biophysica Acta·Julian A Guttman, B Brett Finlay
Jul 23, 2011·Journal of Integrative Bioinformatics·Ke LinJack A M Leunissen
Oct 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stephen R PiccoloW Evan Johnson
Feb 11, 2015·The EMBO Journal·Yaíma L LightfootMansour Mohamadzadeh
Jan 1, 2015·F1000Research·Anand Prakash Singh, Saima Aijaz
Oct 21, 2016·Clinical and Translational Gastroenterology·Julia KönigRobert-Jan Brummer
Feb 24, 2017·Journal of Pediatric Gastroenterology and Nutrition·Shuangshuang GuoKriston Ganguli
Jul 30, 2017·Scientific Reports·Prashanna Balaji VenkatasubramanianJurriaan J Mes
Sep 25, 2017·International Journal of Medical Microbiology : IJMM·Kaiyi ZhangMathias Hornef
Dec 12, 2017·EFSA Journal·UNKNOWN European Food Safety Authority, UNKNOWN European Centre for Disease Prevention and Control

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Citations

Dec 24, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Ling YangXiaoying Zhang
May 6, 2020·Critical Reviews in Food Science and Nutrition·Chengcheng RenPaul de Vos
Feb 6, 2020·Frontiers in Neuroscience·Shin Jie YongWei Ling Lim
Apr 4, 2021·International Journal of Molecular Sciences·Tracy ParadisFabienne Bon

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Datasets Mentioned

BETA
GSE115022

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS Statistics
LIMMA
GraphPad Prism
Bioconductor UPC
SPSS
Ingenuity
Bioconductor
GraphPad

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