Lactobacillus crispatus accelerates re-epithelialization in vaginal epithelial cell line MS74

American Journal of Reproductive Immunology : AJRI
Kazuhide TakadaSatoshi Hayakawa

Abstract

The functions of vaginal lactobacilli in susceptibility to infectious diseases as regards epithelial barrier integrity and wound healing remain incompletely understood. Lactobacillus crispatus, one of the most common Lactobacillus species in the vagina and among the most protective against sexually transmitted infections, was cocultured with an immortalized human vaginal epithelial cell line (MS74), and a scratch assay was performed to evaluate re-epithelialization. The concentration of vascular endothelial growth factor A (VEGF) was measured using enzyme-linked immunosorbent assay (ELISA). An immunofluorescence assay was performed to locate the expression of VEGF and VEGF receptor (VEGFR) 1 and 2. The effects of the bacterial supernatant of L. crispatus were also evaluated. Lactobacillus crispatus significantly accelerated re-epithelialization of MS74 cells, accompanied by an increase in VEGF concentration. In contrast, heat-killed L. crispatus did not show this effect. The bacterial supernatant of L. crispatus also induced re-epithelialization. The immunoreactivity of VEGF was higher at the scratched edge, whereas VEGFR1 and 2 stained site-independently. Recombinant VEGF induced cell migration in a dose-dependent manner. The ...Continue Reading

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Citations

Jun 18, 2020·Immunological Medicine·Shihoko Komine-AizawaSatoshi Hayakawa
Oct 31, 2020·Biotechnology and Applied Biochemistry·Mahnaz MohtashamiAhmad Ghasemi
Dec 29, 2020·Frontiers in Cellular and Infection Microbiology·Yupei XieTingtao Chen
May 20, 2021·MBio·Hannah M Rowe, Jason W Rosch
Apr 28, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Michelle ShardellRebecca M Brotman

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