Lactobacillus fermentum NCIMB 5221 and NCIMB 2797 as cholesterol-lowering probiotic biotherapeutics: in vitro analysis

Beneficial Microbes
Catherine Tomaro-DuchesneauS Prakash

Abstract

Cardiovascular and coronary artery disease risk are correlated with cholesterol levels and are significant health concerns. Current cholesterol-lowering approaches includes lifestyle and diet modifications, as well as statins which presents numerous shortcomings. The probiotic bacteria, Lactobacillus fermentum NCIMB 5221 and NCIMB 2797, have demonstrated cholesterol-lowering potential in animal studies. However, there is a lack in understanding the mechanism(s) behind these observed effects. The goal of this work is to investigate, in vitro, the cholesterol-lowering mechanisms of these two strains. To determine the cholesterol-lowering mechanisms, probiotic cholesterol assimilation, colon epithelial adhesion and inhibition of cholesterol uptake by colon epithelial (Caco-2) cells were investigated. L. fermentum NCIMB 2797 (P=0.012) and NCIMB 5221 (P=0.003) assimilated cholesterol and their cell surface hydrophobicity was 70.30±8.85% and 55.60±2.59%, respectively. Both L. fermentum strains showed no significant impact (P>0.05) on Caco-2 cell viability. Of most interest, Caco-2 pre-exposure to L. fermentum NCIMB 5221 significantly decreased (P=0.015) cholesterol uptake, with 85.98±2.07% uptake compared to the untreated cells. Simi...Continue Reading

References

Aug 11, 1990·BMJ : British Medical Journal·M F MuldoonK A Matthews
Feb 1, 1985·Applied and Environmental Microbiology·S E GillilandC Maxwell
Nov 1, 1983·Infection and Immunity·M RosenbergE Weiss
Apr 1, 1997·Journal of Internal Medicine·W Insull
Aug 30, 2002·Applied and Environmental Microbiology·Dora I A Pereira, Glenn R Gibson
Dec 17, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hae Kyung KimMyung-Sook Choi
Dec 27, 2002·The Journal of General and Applied Microbiology·Virginia S. OcanaMaría Elena Nader-Macias
May 8, 2007·Applied Microbiology and Biotechnology·Jasmine BhathenaSatya Prakash
Jun 19, 2009·The Canadian Journal of Cardiology·Jean-Eric TarrideRon Goeree
Feb 16, 2011·Nature Reviews. Cardiology·Ariel Brautbar, Christie M Ballantyne
Oct 25, 2011·Letters in Applied Microbiology·M C Abeijón MukdsiR B Medina
Dec 27, 2011·The Indian Journal of Medical Research·Raj Kumar DuarySunita Grover
Jun 14, 2012·Expert Opinion on Biological Therapy·Shyamali SahaSatya Prakash
Oct 15, 2013·Applied Microbiology and Biotechnology·Catherine Tomaro-DuchesneauSatya Prakash
Mar 29, 2014·Probiotics and Antimicrobial Proteins·Trine Eker ChristoffersenCharlotte Ramstad Kleiveland

❮ Previous
Next ❯

Citations

Jul 24, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Umar Haris IqbalSatya Prakash
Nov 16, 2019·Critical Reviews in Food Science and Nutrition·Karim NaghmouchiDjamel Drider
Oct 16, 2019·Journal of Medical Microbiology·Kanwal AzizMuhammad Tariq
Aug 23, 2019·Probiotics and Antimicrobial Proteins·Sasikumar Arunachalam PalaniyandiSeung Hwan Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.