Lactobacillus plantarum CUL66 can impact cholesterol homeostasis in Caco-2 enterocytes

Beneficial Microbes
D R MichaelD P Ramji

Abstract

Hypercholesterolemia drives the development of cardiovascular disease, the leading cause of mortality in western society. Supplementation with probiotics that interfere with cholesterol metabolism may provide a contribution to disease prevention. Lactobacillus plantarum CUL66 (NCIMB 30280) has been assessed in vitro for its ability to impact cholesterol absorption. L. plantarum CUL66 tested positive for bile salt hydrolase activity and the ability to assimilate cholesterol from culture media. RT-qPCR analysis showed that the bacterium significantly decreased the expression of Niemann-Pick C1-like 1 and ATP-binding cassette transporter-1 in polarised Caco-2 cells after 6 h exposure. Conversely, the expression of ATP-binding cassette sub-family G member (ABCG)-5 and ABCG-8, and 3-hydroxy-3-methylglutaryl-CoA reductase were significantly increased. Using a radiolabelled assay, we also observed significant reductions in the uptake and basolateral efflux of cholesterol by Caco-2 cells exposed to L. plantarum CUL66. This in vitro study identified L. plantarum CUL66 as a cholesterol lowering bacteria by highlighting its ability to beneficially regulate multiple in vitro events associated with intestinal cholesterol metabolism and prov...Continue Reading

References

Dec 1, 1975·Preventive Medicine·M Kalb
May 1, 1974·The American Journal of Clinical Nutrition·G V Mann
Feb 1, 1996·Journal of Korean Medical Science·M S Lee
Jan 12, 2000·Journal of Dairy Science·H TanakaI Mierau
May 29, 2002·Biochimica Et Biophysica Acta·Ikuo IkedaKatsumi Imaizumi
Aug 15, 2002·Biochemical and Biophysical Research Communications·Tohru OhamaYuji Matsuzawa
Aug 30, 2002·Applied and Environmental Microbiology·Dora I A Pereira, Glenn R Gibson
Sep 19, 2002·Critical Reviews in Biochemistry and Molecular Biology·Dora I A Pereira, Glenn R Gibson
Oct 9, 2002·Circulation·Thomas SudhopKlaus von Bergmann
Feb 21, 2004·Science·Scott W AltmannMichael P Graziano
Dec 14, 2004·Journal of Dairy Science·M T Liong, N P Shah
Nov 30, 2006·Journal of Lipid Research·Dawn E TelfordMurray W Huff
Jan 16, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Harry R DavisScott W Altmann
Aug 11, 2007·Bioscience, Biotechnology, and Biochemistry·Shizuko TachibanaRyuichiro Sato
Aug 23, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mark A ValasekStephen D Turley
Jan 25, 2011·Cardiology Clinics·Joel A Lardizabal, Prakash Deedwania
May 24, 2011·Progress in Lipid Research·James E McLarenDipak P Ramji
Sep 21, 2011·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Z GuoW Chen
Apr 3, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Manuela NatoliArmando Felsani
Apr 24, 2012·Journal of Lipid Research·Luke J EngelkingGuosheng Liang
Mar 5, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L AlemanyA Alegría
Nov 28, 2013·European Heart Journal·Jennifer Taylor
Sep 4, 2014·Asian Pacific Journal of Tropical Biomedicine·Shu Chen WangPin-Der Duh
Jun 4, 2015·The New England Journal of Medicine·Christopher P CannonUNKNOWN IMPROVE-IT Investigators
Aug 12, 2015·The Journal of Cardiovascular Nursing·Catherine Christie

❮ Previous
Next ❯

Citations

Mar 5, 2019·Beneficial Microbes·D R MichaelS F Plummer
May 1, 2020·Bioscience Reports·Bo ZhangChunsheng Li
Feb 6, 2020·Current Microbiology·Aditi Khare, Smriti Gaur
Aug 23, 2020·International Journal of Molecular Sciences·Lee-Ching LewMin-Tze Liong
Apr 21, 2020·Biological Procedures Online·Verena HieblVerena M Dirsch
Aug 16, 2021·Genomics·L M BakerP D Facey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.