Lactobacillus plantarum Lipoteichoic Acid Inhibits Oral Multispecies Biofilm

Journal of Endodontics
A Reum KimSeung Hyun Han

Abstract

Apical periodontitis is an inflammatory disease in the periradicular region of teeth that results from infection by multispecies bacterial biofilm residing in the root canal system. In this study, we investigated whether Lactobacillus plantarum lipoteichoic acid (Lp.LTA) could inhibit multispecies oral pathogenic bacterial biofilm formation. Highly pure and structurally intact Lp.LTA was purified from L. plantarum. Actinomyces naeslundii, Lactobacillus salivarius, Streptococcus mutans, and Enterococcus faecalis were co-cultured to form oral multispecies biofilm in the presence or absence of Lp.LTA on culture plates or human dentin slices. Preformed biofilm was treated with or without Lp.LTA, followed by additional treatment with intracanal medicaments such as calcium hydroxide or chlorhexidine digluconate. Confocal microscopy and crystal violet assay were performed to determine biofilm formation. Biofilm on human dentin slices was visualized with a scanning electron microscope. Biofilm formation of multispecies bacteria on the culture dishes was dose-dependently reduced by Lp.LTA compared with the nontreatment control group. Lp.LTA also inhibited multispecies biofilm formation on the dentin slices in a dose-dependent manner. In...Continue Reading

Citations

Jan 29, 2020·The Journal of Microbiology·A Reum KimSeung Hyun Han
Aug 21, 2020·Microbial Cell Factories·Basavaprabhu H NatarajHariom Yadav
Nov 9, 2020·Microbial Cell Factories·Wallace Jeng Yang CheeLeslie Thian Lung Than
Feb 14, 2021·Probiotics and Antimicrobial Proteins·Gaurav KumarSantosh Kumar Tiwari
Mar 9, 2021·Frontiers in Cellular and Infection Microbiology·Yuanhui HeHuixia Yang
Mar 11, 2021·Periodontology 2000·Nicholas S JakubovicsFabian Cieplik
Jun 10, 2021·Acta Odontologica Scandinavica·Gabriela Ceccon ChiancaNatalia Lopes Pontes Póvoa Iorio

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