Abstract
This study reports the probiotic attributes of Lactobacillus strains isolated from chicken faeces and mainly their capabilities to prevent infectious diseases and improve chicken production performance. Thus, 22 Lactobacillus strains were isolated from 50 chickens' faeces samples and assessed for their resistance to gastric acidity (pH 0.5, 1, 1.5, 2 and 2.5), tolerance to bile salts, adherence to broiler intestinal cells and antibacterial activity. These in vitro screening analyses revealed Lactobacillus plantarum S22 and L. plantarum S27 as the only strains capable to survive at pH 2.0 in MRS broth (log10 cfu/ml=5.02 and 8.46 log respectively), while the remaining strains were not resistant to pH≤2.0. Similarly, 21 strains were resistant to bile at 0.5% (log10 cfu/ml=0.09-3.32 log), but only Lactobacillus fermentum S26, L. plantarum S22 and L. plantarum S27 were able to grow in the presence of 0.1% (w/v) bile (8.23±0.15; 8.39±0.17 and 8.57±0.07 respectively). Most of these isolates (19/22) were active against Escherichia coli ATCC 25922, E. coli SL2016 and Salmonella enterica CIP 81-3. Lactic acid is likely the main antibacterial compound produced since the neutralised supernatant was devoid of any antibacterial activity. In ...Continue Reading
References
Jul 1, 1989·Applied and Environmental Microbiology·G W TannockS D Feighner
Jan 19, 1973·Nature·E Nurmi, M Rantala
Oct 1, 1983·The Journal of Applied Bacteriology·A Mäyrä-MäkinenH Gyllenberg
Sep 29, 1998·Journal of Applied Microbiology·S EnnaharC Hasselmann
Sep 29, 1998·Letters in Applied Microbiology·L Z JinS Jalaludin
Nov 4, 1998·British Poultry Science·V CavazzoniC Castrovilli
Nov 5, 1999·Applied and Environmental Microbiology·C N JacobsenM Jakobsen
Jun 1, 2000·Applied and Environmental Microbiology·P W van Der WielenF van Knapen
Dec 19, 2000·Journal of Applied Microbiology·G KleinG Reuter
Apr 26, 2002·Journal of Applied Microbiology·M A EhrmannR F Vogel
May 20, 2005·Current Microbiology·Susana DelgadoBaltasar Mayo
Jul 18, 2006·Research in Microbiology·Maria De AngelisMarco Gobbetti
Dec 1, 2006·Applied Microbiology and Biotechnology·Pyoung Il KimYong-Ha Park
Oct 24, 2007·Poultry Science·J I R Castanon
Dec 24, 2008·Animal Health Research Reviews·T R CallawayD J Nisbet
Jan 30, 2010·International Journal of Molecular Sciences·S M Lutful Kabir
Oct 19, 2010·Anaerobe·Chung-Yi WangYuan-Tay Shyu
Mar 31, 2011·BMC Microbiology·Erwann HamonSaïd Ennahar
Nov 6, 2012·Anaerobe·S MessaoudiN Connil
Mar 12, 2013·Trends in Microbiology·Thaddeus B Stanton
Dec 3, 2013·Anaerobe·Dobroslava BujnakovaVladimir Kmet
Sep 23, 2014·Poultry Science·Megan A SpiveyTri Duong
Oct 9, 2014·Brazilian Journal of Biology = Revista Brasleira De Biologia·D J SilvaG R Canale
Mar 7, 2015·Poultry Science·Sandra Diaz-SanchezIrene Hanning
Feb 2, 2018·The Lancet. Planetary Health·Karen L TangWilliam A Ghali
Feb 8, 2018·Expert Review of Proteomics·Markus Kostrzewa
Mar 24, 2018·Research in Veterinary Science·Marcin SmialekAndrzej Koncicki
May 17, 2018·Animal Nutrition·Shengfa F Liao, Martin Nyachoti
Oct 24, 2018·Infection and Drug Resistance·Bilal AslamZulqarnain Baloch