PMID: 18716170Aug 22, 2008Paper

Lactobacillus reuteri 100-23 transiently activates intestinal epithelial cells of mice that have a complex microbiota during early stages of colonization

The Journal of Nutrition
Micha HoffmannDirk Haller

Abstract

Monoassociations of germ-free animals with colitogenic and probiotic bacterial strains trigger intestinal epithelial cell (IEC) activation and host-derived feedback mechanisms. To characterize the impact of a single nonpathogenic bacterial strain on the intestinal epithelium in the presence of an established microbiota, we inoculated reconstituted Lacotobacillus-free (RLF) mice at 8 wk of age with Lactobacillus reuteri 100-23. Primary IEC from the small intestine of L. reuteri-inoculated and control RLF mice were isolated 2, 6, and 21 d after inoculation followed by gene expression analysis (real-time PCR; Affymetrix microarrays) as well as 2-dimensional-gel electrophoreses (2D SDS-PAGE) and peptide mass fingerprinting via matrix-assisted laser desorption/ionization time of flight MS. At d 6, gene expression of proinflammatory cytokines and chemokines including interleukin (IL)-1alpha, IL-6, interferon-gamma-inducible protein 10, and macrophage inflammatory protein 2 was transiently induced, whereas gene expression levels of regulatory proteins A20 and Toll-interacting protein decreased. In addition, 8 target proteins with changes in the steady-state protein expression levels were identified at d 2 and 6 of L. reuteri colonizat...Continue Reading

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Citations

Dec 29, 2010·International Journal of Inflammation·Gerald W Tannock
Jan 21, 2012·The British Journal of Nutrition·Linda V Thomas, Theo Ockhuizen
Jul 16, 2013·Applied and Environmental Microbiology·Jovanka LukicJelena Begovic
May 9, 2015·Journal of Animal Science and Biotechnology·Chengli HouShiyan Qiao
Dec 4, 2009·International Reviews of Immunology·Yolanda Sanz, Giada De Palma
Dec 8, 2010·Pediatric Research·Natalie S DykstraDavid R Mack
Sep 30, 2009·Immunology and Cell Biology·Megan LivingstonMargaret Baird
Nov 25, 2016·Molecular Nutrition & Food Research·Amel TaibiElena M Comelli
Feb 18, 2011·Gut Microbes·Mary Ellen SandersElaine Vaughan
Sep 2, 2011·The Journal of Nutrition·Swantje C DunckerJohn Bienenstock
May 16, 2014·Microbial Ecology·Jovanka LukicJelena Begovic
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jens WalterStefan Roos

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