Lactobacillus reuteri extracts promoted wound healing via PI3K/AKT/β-catenin/TGFβ1 pathway

Stem Cell Research & Therapy
Nannan HanYi Liu

Abstract

The balance of oral microbiomes is crucial to maintain oral health. Microecological imbalance can impair the function of mesenchymal stem cells (MSCs) and lead to delay wound healing. Probiotics is a promising prevention approach for the treatment of oral inflammatory diseases caused by a bacterial infection. However, the effect of probiotics on oral MSCs and wound healing is unclear. In the present study, we used one type of probiotics Lactobacillus reuteri extracts to determine whether bacterial extracts could regulate the functions of gingiva MSCs (GMSCs) and promote wound healing. Lactobacillus reuteri was prepared with bacterial extracts using ultrasonic crushing apparatus. The effects of Lactobacillus reuteri extracts on GMSCs were tested using the cell scratch migration, alkaline phosphatase (ALP) activity, alizarin red staining, cell counting kit-8, real-time PCR, and western blot assays. To investigate the role of Lactobacillus reuteri extracts in the wound in mice, the wound position of bilateral mesial gingival of the maxillary first molar was established, the wound area with a size of 1 mm × 2 mm and the full thickness gingiva was removed. Mice with wound were randomly distributed to two groups: injection of 0.9% Na...Continue Reading

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Citations

Jun 5, 2020·Artificial Cells, Nanomedicine, and Biotechnology·Lei QiLei Zhang
Apr 27, 2020·BMC Oral Health·Riham Hadj-HamouEleftherios G Kaklamanos
Oct 31, 2020·Biotechnology and Applied Biochemistry·Mahnaz MohtashamiAhmad Ghasemi
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Mar 25, 2021·Expert Opinion on Drug Delivery·Anže ZidarŠpela Zupančič
Oct 1, 2021·Frontiers in Cellular and Infection Microbiology·Rachael M WilsonKingsley Yin

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Methods Mentioned

BETA
fluorescence microscopy
PCR
biopsies

Software Mentioned

ImageJ
Pro
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