Lactobacillus reuteri suppresses E. coli O157:H7 in bovine ruminal fluid: Toward a pre-slaughter strategy to improve food safety?

PloS One
Yolande BertinEvelyne Forano

Abstract

The bovine gastrointestinal tract (GIT) is the main reservoir for enterohaemorrhagic Escherichia coli (EHEC) responsible for food-borne infections. Therefore, it is crucial to develop strategies, such as EHEC suppression by antagonistic microorganisms, to reduce EHEC survival in the GIT of cattle and to limit shedding and food contamination. Most human-derived Lactobacillus reuteri strains produce hydroxypropionaldehyde (HPA), an antimicrobial compound, during anaerobic reduction of glycerol. The capacity of L. reuteri LB1-7, a strain isolated from raw bovine milk, to produce HPA and its antimicrobial activity against an O157:H7 EHEC strain (FCH6) were evaluated in bovine rumen fluid (RF) under strict anaerobiosis. EHEC was totally suppressed when incubated in RF inoculated with L. reuteri LB1-7 and supplemented with 80 mM glycerol (RF-Glyc80). The addition of LB1-7 or glycerol alone did not modify EHEC survival in RF. Glycerol was converted to HPA (up to 14 mM) by LB1-7 during incubation in RF-Glyc80, and HPA production appeared to be responsible for EHEC suppression. The bactericidal activity of L. reuteri LB1-7, the concentration of glycerol required and the level of HPA produced depended on physiological and ecological envi...Continue Reading

References

Jul 1, 1991·The Veterinary Clinics of North America. Food Animal Practice·W H Hoover, T K Miller
May 1, 1989·Antimicrobial Agents and Chemotherapy·T L Talarico, W J Dobrogosz
Jan 1, 1997·Applied and Environmental Microbiology·C A BrownM P Doyle
Aug 1, 1997·Journal of Animal Science·M D SternS Calsamiglia
Oct 26, 1999·Applied Microbiology and Biotechnology·H BieblW D Deckwer
Oct 17, 2002·Applied Microbiology and Biotechnology·Q Lüthi-PengZ Puhan
May 15, 2003·Journal of Agricultural and Food Chemistry·Sabine VollenweiderZdenko Puhan
Jun 1, 1961·Journal of General Microbiology·G A GARTONE VIOQUE
Jun 1, 1961·Journal of General Microbiology·P N HOBSON, S O MANN
Apr 23, 2005·Veterinary Research·Alfredo CaprioliEric Oswald
Jun 6, 2006·Applied and Environmental Microbiology·Frédérique Chaucheyras-DurandChristine Martin
Nov 11, 2009·Foodborne Pathogens and Disease·Callie WalkerT G Nagaraja
Apr 13, 2010·International Journal of Food Microbiology·Francesca GaggìaBruno Biavati
Sep 14, 2010·Applied and Environmental Microbiology·Liv Anette BøhleIngolf F Nes
Sep 18, 2010·Journal of Agricultural and Food Chemistry·Sabine VollenweiderChristophe Lacroix
Sep 21, 2010·Applied and Environmental Microbiology·Lucia RivasGeraldine Duffy
Mar 1, 2010·Beneficial Microbes·F Chaucheyras-Durand, H Durand
Nov 7, 2012·Environmental Microbiology·Yolande BertinChristine Martin
Jul 9, 2014·Annual Review of Microbiology·Bryan A WhiteHarry J Flint
May 21, 2015·Journal of International Society of Preventive & Community Dentistry·Vijayendra PandeyNarender Singh Malik

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Sequences Nucleotide BLAST
GraphPad Instat
Align

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