Lactobacillus rhamnosus BFE 5264 and Lactobacillus plantarum NR74 Promote Cholesterol Excretion Through the Up-Regulation of ABCG5/8 in Caco-2 Cells

Probiotics and Antimicrobial Proteins
Hongsup YoonWilhelm H Holzapfel

Abstract

The effect of two putative probiotic strains, Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74, on the control of cholesterol efflux in enterocytes was assessed by focusing on the promotion of ATP-binding cassette sub-family G members 5 and 8 (ABCG5 and ABCG8). Differentiated Caco-2 enterocytes were treated with live bacteria, heat-killed bacteria, a bacterial cell wall fraction, and metabolites and were subjected to cholesterol uptake assay, mRNA analysis, and protein analyses. Following LXR-transfection by incubation with CHO-K1 cells in DNA-lipofectin added media, the luciferase assay was conducted for LXR analysis. Treatment of Caco-2 cells with L. rhamnosus BFE5264 (isolated from traditional fermented Maasai milk) and L. plantarum NR74 (isolated from Korean kimchi) resulted in the up-regulation of LXR, concomitantly with the elevated expression of ABCG5 and ABCG8. This was associated with the promotion of cholesterol efflux at significantly higher levels compared to the positive control strain L. rhamnosus GG (LGG). The experiment with CHO-K1 cells confirmed up-regulation of LXR-beta by the test strains, and treatment with the live L. rhamnosus BFE5264 and L. plantarum NR74 strains significantly increased c...Continue Reading

References

May 1, 1974·The American Journal of Clinical Nutrition·G V Mann
Jul 1, 1995·Journal of Dairy Science·G PerdigonN Gobbato
Feb 14, 1998·Journal of Cellular Physiology·M J EngleD H Alpers
Mar 6, 1999·Journal of the American College of Nutrition·J W Anderson, S E Gilliland
Jan 12, 2000·Journal of Dairy Science·H TanakaI Mierau
Apr 4, 2000·European Journal of Clinical Nutrition·L Agerholm-LarsenA Astrup
Sep 1, 2000·FEMS Immunology and Medical Microbiology·H FangS Seppo
Jan 11, 2000·European Journal of Clinical Nutrition·L Agerholm-LarsenA Astrup
Mar 20, 2002·The Journal of Biological Chemistry·Joyce J RepaDavid J Mangelsdorf
Nov 18, 2003·Journal of Lipid Research·Lei CaiDeneys R van der Westhuyzen
Sep 10, 2004·Postgraduate Medical Journal·H S Gill, F Guarner
Dec 14, 2004·Journal of Dairy Science·M T Liong, N P Shah
Mar 2, 2005·Pharmacology & Therapeutics·Thomas SudhopKlaus von Bergmann
Apr 16, 2005·Current Opinion in Biotechnology·Maija SaxelinWillem M de Vos
Jul 19, 2005·Journal of Lipid Research·Steven L SabolSilvia Santamarina-Fojo
Jan 28, 2006·Pflügers Archiv : European journal of physiology·Starr E Hazard, Shailendra B Patel
Mar 3, 2006·Current Opinion in Biotechnology·Maria L MarcoMichiel Kleerebezem
Mar 3, 2006·The Journal of Clinical Investigation·Noam Zelcer, Peter Tontonoz
Jan 5, 2007·Experimental & Molecular Medicine·Bong-Hyuk ChoiMyoung-Sool Do
Jul 28, 2007·The Journal of Clinical Investigation·Michelle N BradleyPeter Tontonoz
Mar 22, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Heleen M de Vogel-van den BoschRoelof van der Meer
Jun 10, 2008·International Journal of Food Microbiology·Julius Maina MatharaWilhelm H Holzapfel
Jun 14, 2008·American Journal of Physiology. Cell Physiology·Han Geun KimDae Kyun Chung
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Sarah LebeerSigrid C J De Keersmaecker
Jun 2, 2009·Microbial Cell Factories·Rob BrooijmansJeroen Hugenholtz
Nov 6, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Esther M E van StratenTorsten Plösch
Feb 23, 2010·Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry·J Mark Brown, Liqing Yu
Jul 20, 2010·International Journal of Molecular Sciences·Lay-Gaik Ooi, Min-Tze Liong
Jan 11, 2011·International Journal of Food Microbiology·Heejae LeeWilhelm Holzapfel

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Citations

Nov 8, 2019·Molecular Nutrition & Food Research·Victoria L O'Morain, Dipak P Ramji
May 1, 2020·Bioscience Reports·Bo ZhangChunsheng Li
Jul 24, 2018·Bioscience, Biotechnology, and Biochemistry·Wan HeoYoung-Jun Kim
Aug 23, 2020·International Journal of Molecular Sciences·Lee-Ching LewMin-Tze Liong
Apr 21, 2020·Biological Procedures Online·Verena HieblVerena M Dirsch
Jun 3, 2021·Microorganisms·Mélanie BourginMoez Rhimi
Oct 24, 2018·Journal of Agricultural and Food Chemistry·Yen-Ning LinYun-Ping Lim
Oct 15, 2021·Probiotics and Antimicrobial Proteins·Hongsup YoonWilhelm H Holzapfel

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