Lactobacillus rhamnosus GG and Lactobacillus casei suppress Escherichia coli-induced chemokine expression in intestinal epithelial cells

International Archives of Allergy and Immunology
Shinji TokiKenji Matsumoto

Abstract

Recently, some strains of lactic acid bacteria (LAB) have been reported to prevent the development of atopic dermatitis and to improve allergic symptoms, especially in young children. However, the mechanisms involved in these effects are not fully understood. Intestinal microbiota play critical roles in the development of host immune development and are recognized and regulated by the host through intestinal epithelial cells (IECs). We thus hypothesized that LAB influence the host immune system through the activation of IECs. To begin testing this hypothesis, chemokine expression in IECs exposed to intestinal bacteria was investigated. Caco-2 cell monolayers were stimulated with different concentrations of various live or heat-killed intestinal bacteria or bacterial components for up to 3 h. Changes in the gene expressions of various chemokines were measured using quantitative real-time PCR. The expressions of CCL20, CXCL8, CXCL10 and CX3CL1 were strongly induced by nonpathogenic Escherichia coli in a dose-dependent manner and were partially induced by some commensal LAB. In contrast, Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei did not induce these chemokine expressions. In addition, LGG significantly suppressed th...Continue Reading

Citations

Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Shengbin RaoFumiki Morimatsu
Mar 22, 2013·Cellular and Molecular Life Sciences : CMLS·Francesca TurroniDouwe van Sinderen
May 12, 2016·Nature Communications·Pranjul ShahPaul Wilmes
Dec 24, 2010·Virulence·Susan R HeimerMichael Gilmore
Jul 7, 2015·Journal of the Science of Food and Agriculture·Yen-Po ChenMing-Ju Chen
Nov 30, 2018·Frontiers in Physiology·Sarah C PearceKenneth Racicot
Oct 17, 2019·Investigative Ophthalmology & Visual Science·Changjun WangCintia S de Paiva

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