Lactobacillus sakei WIKIM30 Ameliorates Atopic Dermatitis-Like Skin Lesions by Inducing Regulatory T Cells and Altering Gut Microbiota Structure in Mice

Frontiers in Immunology
Min-Sung KwonHak-Jong Choi

Abstract

Lactobacillus sakei WIKIM30 is a Gram-positive facultative anaerobic bacterium isolated from kimchi, a Korean fermented vegetable food. In this study, we found that WIKIM30 promoted regulatory T cell (Treg) differentiation by inducing dendritic cells with tolerogenic properties. The production of the T helper (Th) 2-associated cytokine interleukin (IL)-4 was decreased, but that of the Treg-associated cytokine IL-10 was increased in splenocytes from ovalbumin-sensitized mice treated with WIKIM30. We also investigated the inhibitory capacity of WIKIM30 on the development of 2,4-dinitrochlorobenzene-induced atopic dermatitis (AD), a Th2-dominant allergic disease in mice. Oral administration of L. sakei WIKIM30 significantly reduced AD-like skin lesions and serum immunoglobulin E and IL-4 levels while decreasing the number of CD4+ T cells and B cells and the levels of Th2 cytokines (IL-4, IL-5, and IL-13) in peripheral lymph nodes and enhancing Treg differentiation and IL-10 secretion in mesenteric lymph nodes. In addition, WIKIM30 modulated gut microbiome profiles that were altered in AD mice, which showed increases in Arthromitus and Ralstonia and a decrease in Ruminococcus abundance. These changes were reversed by WIKIM30 treatm...Continue Reading

References

Apr 29, 1998·International Journal of Food Microbiology·F Guarner, G J Schaafsma
Mar 17, 2000·Immunological Reviews·R Medzhitov, C Janeway
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M WhelanK P Rigley
Dec 29, 2000·Nature·H HemmiS Akira
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B PulendranJ Banchereau
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanne R ChristensenJames J Pestka
Jan 18, 2003·Lancet·Donald Y M Leung, Thomas Bieber
Mar 19, 2003·The Journal of Allergy and Clinical Immunology·Shinichi WatanabeMegumi Kumemura
Oct 26, 2005·Trends in Immunology·Jochen Huehn, Alf Hamann
Jul 4, 2006·The Journal of Allergy and Clinical Immunology·Bernhard HomeyDonald Y M Leung
Oct 20, 2006·Current Topics in Microbiology and Immunology·R M Steinman, H Hemmi
Jun 21, 2008·Nature Reviews. Immunology·Dario A A VignaliCreg J Workman
Aug 20, 2008·The Journal of Experimental Medicine·Elin JaenssonWilliam W Agace
Apr 1, 2009·PloS One·Salim BouguermouhMarika Sarfati
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ho-Keun KwonSin-Hyeog Im
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yun Kyung LeeSarkis K Mazmanian
Sep 18, 2010·Inflammatory Bowel Diseases·Seungha KangChris S McSweeney
Feb 18, 2011·Gut Microbes·Sarah O'FlahertyJames Versalovic
Jun 21, 2011·Immunological Reviews·Mark Boguniewicz, Donald Y M Leung
Oct 14, 2011·Journal of Clinical & Cellular Immunology·Eric B Brandt, Umasundari Sivaprasad
Feb 7, 2012·Trends in Immunology·Yaozhong DingJonathan S Bromberg
Jan 1, 2011·Frontiers in Immunology·Darren Thomas Ruane, Ed C Lavelle
Aug 21, 2013·Trends in Immunology·Warren Strober
Jan 23, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W Peng, N Novak
Mar 31, 2015·The Journal of Allergy and Clinical Immunology·Vijay R RamakrishnanDaniel N Frank
May 15, 2015·Cell Host & Microbe·Kei E Fujimura, Susan V Lynch
Jan 1, 2014·Central-European Journal of Immunology·Wioletta Żukiewicz-SobczakWojciech Silny
Jul 17, 2015·Movement Disorders : Official Journal of the Movement Disorder Society·Ali KeshavarzianKathleen M Shannon
Oct 4, 2015·The Journal of Allergy and Clinical Immunology·Han SongHeenam Stanley Kim
May 11, 2016·Journal of Human Nutrition and Dietetics : the Official Journal of the British Dietetic Association·C MilaniM Ventura
Aug 3, 2016·Scientific Reports·Xiaofei LiuYongfei Fang
Mar 16, 2017·Scientific Reports·Line Fisker ZachariassenAxel Kornerup Hansen

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Citations

Sep 15, 2019·Journal of the European Academy of Dermatology and Venereology : JEADV·B Polkowska-PruszyńskaD Krasowska
Jan 17, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiaorui ZhouWenzhe Li
Aug 5, 2020·Applied Microbiology and Biotechnology·Se Hee LeeChe Ok Jeon
Jul 7, 2020·Microbes and Environments·Jiayue YangShinji Fukuda
Mar 19, 2020·Frontiers in Immunology·Tasuku OgitaTakeshi Shimosato
May 1, 2021·International Journal of Molecular Sciences·Dong Hoon ParkDae-Hyun Hahm
May 6, 2021·Nutrients·Roghayeh ShahbaziChantal Matar
May 22, 2021·Food Science of Animal Resources·Hayoung KimYounghoon Kim
Jul 6, 2021·Frontiers in Veterinary Science·Ling ZhangJinhua Zhang
Oct 12, 2021·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Jae-Rin AhnSoo-Jong Hong

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Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assay
amplicon sequencing

Software Mentioned

BIOiPLUG
Prism
XLSTAT
GraphPad
FlowJo

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