Lactococcal 949 group phages recognize a carbohydrate receptor on the host cell surface

Applied and Environmental Microbiology
Jennifer MahonyDouwe van Sinderen

Abstract

Lactococcal bacteriophages represent one of the leading causes of dairy fermentation failure and product inconsistencies. A new member of the lactococcal 949 phage group, named WRP3, was isolated from cheese whey from a Sicilian factory in 2011. The genome sequence of this phage was determined, and it constitutes the largest lactococcal phage genome currently known, at 130,008 bp. Detailed bioinformatic analysis of the genomic region encoding the presumed initiator complex and baseplate of WRP3 has aided in the functional assignment of several open reading frames (ORFs), particularly that for the receptor binding protein required for host recognition. Furthermore, we demonstrate that the 949 phages target cell wall phospho-polysaccharides as their receptors, accounting for the specificity of the interactions of these phages with their lactococcal hosts. Such information may ultimately aid in the identification of strains/strain blends that do not present the necessary saccharidic target for infection by these problematic phages.

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Citations

Jan 7, 2016·Annual Review of Food Science and Technology·Jennifer MahonyDouwe van Sinderen
Mar 11, 2018·Applied and Environmental Microbiology·Katherine LavelleJennifer Mahony
Aug 28, 2018·Journal of Basic Microbiology·Ping LiJingxue Wang
Jun 19, 2016·Applied and Environmental Microbiology·Brian McDonnellDouwe van Sinderen
Feb 26, 2020·Infection and Immunity·Mihnea R Mangalea, Breck A Duerkop
Aug 24, 2017·FEMS Microbiology Reviews·Jennifer MahonyDouwe van Sinderen
Aug 15, 2018·Nature Reviews. Microbiology·Franklin L NobregaStan J J Brouns
Feb 12, 2017·BMC Genomics·Jennifer MahonyDouwe van Sinderen
Apr 26, 2019·Frontiers in Microbiology·Philip KelleherDouwe van Sinderen
Mar 1, 2019·Frontiers in Microbiology·Kai-Yue ZhangFeng-Biao Guo
Aug 12, 2021·Computational and Structural Biotechnology Journal·Katherine LavelleJennifer Mahony

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