Lactococcus lactis FNBPA+ (pValac:e6ag85a) Induces Cellular and Humoral Immune Responses After Oral Immunization of Mice.

Frontiers in Microbiology
Camila Prósperi de CastroAnderson Miyoshi

Abstract

The development of a new vaccine strategy against tuberculosis is urgently needed and has been greatly encouraged by the scientific community worldwide. In this work, we constructed a lactococcal DNA vaccine based on the fusion of two Mycobacterium tuberculosis antigens, ESAT-6 and Ag85A, and examined its immunogenicity. The coding sequences of the ESAT-6 and Ag85A genes were fused and cloned into the eukaryotic expression pValac vector, and the functionality of the vector was confirmed in vitro. Then, L. lactis FnBPA+ (pValac:e6ag85a) was obtained and used for oral immunization of mice. This strain induced significant increases in IFN-γ, TNF-α, and IL-17 cytokines in stimulated splenocyte cultures, and significant production of antigen-specific sIgA was observed in the colonic tissues of immunized mice. We demonstrated that L. lactis FnBPA+ (pValac:e6ag85a) generated a cellular and humoral immune response after oral immunization of mice. The strategy developed in this work may represent an interesting DNA mucosal vaccine candidate against tuberculosis, using the fusion of two highly immunogenic antigens delivered by safe lactic acid bacteria.

References

Jun 1, 1991·Infection and Immunity·P AndersenI Heron
Dec 1, 1993·The Journal of Experimental Medicine·A M CooperI M Orme
Sep 1, 1993·Journal of Bacteriology·P LangellaA Gruss
Feb 29, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·M JacobsB Ryffel
Mar 13, 2001·Annual Review of Immunology·M Feldmann, R N Maini
Sep 5, 2002·Research in Veterinary Science·Stephen P Dunham
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheng-Yuan KaoReen Wu
Nov 10, 2004·International Journal of Medical Microbiology : IJMM·Christoph SchoenSabine Pilgrim
Nov 30, 2004·The Lancet Infectious Diseases·Esther van de VosseTom H M Ottenhoff
May 9, 2006·Tuberculosis·Jes DietrichPeter Andersen
Sep 11, 2008·Nature Reviews. Genetics·Michele A Kutzler, David B Weiner
Feb 12, 2009·Genetic Vaccines and Therapy·Valeria GuimarãesAnderson Miyoshi
Mar 12, 2009·Inflammation & Allergy Drug Targets·Amanda MootooRajko Reljic
May 25, 2010·Clinical and Experimental Immunology·J Harris, J Keane
Dec 2, 2011·Annual Review of Food Science and Technology·Jerry Wells
Jul 23, 2013·Frontiers in Immunology·Blaise Corthésy
Apr 11, 2014·The Lancet. Respiratory Medicine·Stefan H E KaufmannMarkus Maeurer
Sep 23, 2014·Nature Reviews. Immunology·Allan M Mowat, William W Agace
Dec 17, 2014·Applied Microbiology and Biotechnology·Vanessa Bastos PereiraAnderson Miyoshi
Feb 27, 2015·Science Translational Medicine·Stefan H E KaufmannWillem A Hanekom
Dec 8, 2015·Mediators of Inflammation·I V Lyadova, A V Panteleev

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Software Mentioned

FCAP array
GraphPad Prism
Zeiss LSM Image Browser

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