Lactococcus lactis V7 inhibits the cell invasion of bovine mammary epithelial cells by Escherichia coli and Staphylococcus aureus

Beneficial Microbes
B Seridan AssisYves Le Loir

Abstract

Bovine mastitis, an inflammatory disease of the mammary gland often associated to bacterial infection, is the first cause of antibiotic use in dairy cattle. Because of the risk of antibioresistance emergence, alternative non-antibiotic strategies are needed to prevent or to cure bovine mastitis and reduce the antibiotic use in veterinary medicine. In this work, we investigated Lactococcus lactis V7, a strain isolated from the mammary gland, as a probiotic option against bovine mastitis. Using bovine mammary epithelial cell (bMEC) culture, and two representative strains for Escherichia coli and for Staphylococcus aureus, two major mastitis pathogens, we investigated L. lactis V7 ability to inhibit cell invasion (i.e. adhesion and internalization) of these pathogens into bMEC. L. lactis V7 ability to modulate the production of CXCL8, a key chemokine IL-8 responsible for neutrophil influx, in bMEC upon challenge with E. coli was investigated by an ELISA dosage of CXCL8 in bMEC culture supernatants. We showed that L. lactis V7 inhibited the internalisation of both E. coli and S. aureus strains into bMEC, whereas it inhibited the adhesion of only one out of the two S. aureus strains and of none of the E. coli strains tested. Investi...Continue Reading

References

Jul 13, 2006·Letters in Applied Microbiology·M C OteroM E Nader-Macías
Feb 3, 2007·Journal of Food Protection·María G Vizoso PintoCharles M A P Franz
May 12, 2009·Applied and Environmental Microbiology·Sergine EvenYves Le Loir
Dec 24, 2010·PloS One·Delphine PasseriniPascal Le Bourgeois
Dec 20, 2011·Veterinary Microbiology·Oudessa Kerro-DegoAndrew A Potter
Nov 28, 2012·Applied and Environmental Microbiology·Damien S BouchardSergine Even
Jan 8, 2013·International Journal of Food Microbiology·Delphine PasseriniMarie-Line Daveran-Mingot
May 18, 2013·Journal of Veterinary Pharmacology and Therapeutics·L SuojalaS Pyörälä
Jun 1, 2011·Environmental Microbiology Reports·Marina CretenetSergine Even
Jul 23, 2013·Applied and Environmental Microbiology·Delphine PasseriniMuriel Cocaign-Bousquet

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Citations

Aug 14, 2019·Critical Reviews in Microbiology·Angeliki AngelopoulouR Paul Ross
Aug 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·V Krömker, S Leimbach
Jul 13, 2019·Microbiology Spectrum·Philippe GauduAlexandra Gruss
Sep 10, 2021·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Raphael S SteinbergÁlvaro C Nunes

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