Jan 1, 1992

Lactoferrin in the mouse uterus: analyses of the preimplantation period and regulation by ovarian steroids

Molecular Endocrinology
M T McMasterG K Andrews

Abstract

Uterine expression of lactoferrin (LF) during the preimplantation period and its regulation by the ovarian steroids estradiol (E2) and/or progesterone (P4) in ovariectomized adult mice were examined. Immunoblot detection of LF in uterine cell lysates demonstrated the presence of this protein from days 1-8 of pregnancy [day 1 (D1) = day of vaginal plug]. Immunoprecipitation of 35S pulse-labeled uterine proteins showed that the relative rate of LF synthesis was high on D1, but below the level of detection by D4. Immunolocalization of LF in uterine sections showed intense luminal and glandular epithelial staining on D1 and D2, and progressively decreased staining through D4. Immunoreactive protein was also detected in cells, primarily concentrated in the stroma. The relative number of these cells was greatest on D1 and decreased progressively to a low number by D4. These cells were morphologically similar to neutrophils, which are known to contain LF protein, but little or no LF mRNA. Northern blotting showed that uterine LF mRNA levels were very high on D1 and D2 of pregnancy and decreased to low, but detectable, levels by D4. In situ hybridization to uterine sections showed that LF mRNA was highly abundant only in glandular and ...Continue Reading

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Mentioned in this Paper

Progesterone, (9 beta,10 alpha)-Isomer
Uterus
Estradiol, (16 alpha,17 beta)-Isomer
Gene Expression
Prenidation Embryo Development, Animal
Bilateral Oophorectomy
Genomic Hybridization
Lactoferrin
Ltf

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