Lactoferrin induces cell surface retention of prion protein and inhibits prion accumulation

Journal of Neurochemistry
Yoshifumi IwamaruHiroyuki Okada

Abstract

Prion diseases are fatal neurodegenerative disorders, and the conformational conversion of normal cellular prion protein (PrP(C)) into its pathogenic, amyloidogenic isoform (PrP(Sc)) is the essential event in the pathogenesis of these diseases. Lactoferrin (LF) is a cationic iron-binding glycoprotein belonging to the transferrin (TF) family, which accumulates in the amyloid deposits in the brain in neurodegenerative disorders, such as Alzheimer's disease and Pick's disease. In the present study, we have examined the effects of LF on PrP(Sc) formation by using cell culture models. Bovine LF inhibited PrP(Sc) accumulation in scrapie-infected cells in a time- and dose-dependent manner, whereas TF was not inhibitory. Bioassays of LF-treated cells demonstrated prolonged incubation periods compared with non-treated cells indicating a reduction of prion infectivity. LF mediated the cell surface retention of PrP(C) by diminishing its internalization and was capable of interacting with PrP(C) in addition to PrP(Sc). Furthermore, LF partially inhibited the formation of protease-resistant PrP as determined by the protein misfolding cyclic amplification assay. Our results suggest that LF has multifunctional antiprion activities.

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Citations

Jun 7, 2013·Journal of Virology·Yoshifumi IwamaruHiroshi Kitani
Jun 14, 2012·PloS One·Yoshifumi IwamaruHiroshi Kitani
Feb 26, 2013·International Journal of Molecular Sciences·Feng-Yun J HuangJem-Mau Lo
Nov 29, 2013·BioMed Research International·Nidhi MishraSumit Arora
Mar 1, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Minyan WeiChuanbin Wu
Jan 12, 2011·Biochemical and Biophysical Research Communications·Taichi HamanakaKatsumi Doh-ura
Dec 15, 2015·Journal of Neurochemistry·Yoshifumi IwamaruTakashi Yokoyama
Jan 13, 2017·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Chuang GuoZhan-You Wang
Dec 12, 2012·International Journal of Molecular Medicine·Yang-Gyu ParkSang-Youel Park
May 5, 2009·Médecine sciences : M/S·Annick PierceJoël Mazurier
Dec 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mahesh G AdimoolamManorama V Sunkara
Aug 8, 2021·Cells·Yu-Qi Li, Chuang Guo

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