Lactoferrin-modified PEGylated liposomes loaded with doxorubicin for targeting delivery to hepatocellular carcinoma

International Journal of Nanomedicine
Minyan WeiChuanbin Wu

Abstract

Lactoferrin (Lf) is a potential-targeting ligand for hepatocellular carcinoma (HCC) cells because of its specific binding with asialoglycoprotein receptor (ASGPR). In this present work, a doxorubicin (DOX)-loaded, Lf-modified, polyethylene glycol (PEG)ylated liposome (Lf-PLS) system was developed, and its targeting effect and antitumor efficacy to HCC was also explored. The DOX-loaded Lf-PLS system had spherical or oval vesicles, with mean particle size approximately 100 nm, and had an encapsulation efficiency of 97%. The confocal microscopy and flow cytometry indicated that the cellular uptake of Lf-PLS was significantly higher than that of PEGylated liposome (PLS) in ASGPR-positive cells (P<0.05) but not in ASGPR-negative cells (P>0.05). Cytotoxicity assay by MTT demonstrated that DOX-loaded Lf-PLS showed significantly stronger antiproliferative effects on ASGPR-positive HCC cells than did PLS without the Lf modification (P<0.05). The in vivo antitumor studies on male BALB/c nude mice bearing HepG2 xenografts demonstrated that DOX-loaded Lf-PLS had significantly stronger antitumor efficacy compared with PLS (P<0.05) and free DOX (P<0.05). All these results demonstrated that a DOX-loaded Lf-PLS might have great potential appli...Continue Reading

Citations

Sep 3, 2016·International Journal of Molecular Sciences·Panoraia I SiafakaDimitrios N Bikiaris
Oct 4, 2019·Current Drug Metabolism·Siyu GuanJihui Tang
Aug 6, 2020·International Journal of Molecular Sciences·Stefano BurgioCeleste Caruso Bavisotto
Jan 1, 2020·Cancers·Sue Anne ChewSerena Danti
Dec 15, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Diana A Ramírez-SánchezNidia León-Sicairos

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Methods Mentioned

BETA
confocal
flow cytometry
gel filtration
dynamic light scattering
confocal microscopy
flow
xenograft
xenografts

Software Mentioned

SPSS

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Carcinoma, Hepatocellular

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