PMID: 9003378Dec 15, 1996Paper

Lactoferrin regulates the activity of heparin proteoglycan-bound mast cell chymase: characterization of the binding of heparin to lactoferrin

The Biochemical Journal
G Pejler

Abstract

Rat mast cell protease 1 (RMCP-1) is a secretory granule serine protease (chymase) that is recovered in vivo in a macromolecular complex with heparin proteoglycan (PG). We have previously shown that heparin activates RMCP-1 and that RMCP-1, when bound to heparin PG, is largely resistant to inhibition by a variety of macromolecular protease inhibitors. In the search for alternative mechanisms in the regulation of RMCP-1 activity, we hypothesized that heparin antagonists, by interfering with the RMCP-1/heparin PG interaction, might influence the activity of heparin-bound mast cell chymase. In the present study, lactoferrin (LF), a heparin-binding protein, was assessed for RMCP-1 inhibiting activity. LF proved to decrease the activity of heparin PG-associated RMCP-1, although a portion of the enzyme activity was resistant to regulation. The mechanism of regulation was shown to involve the displacement of RMCP-1 from heparin PG, and LF caused an approx. 6-fold increase in the apparent Km of the RMCP-1-heparin PG complex for the chromogenic substrate S-2586. The interaction of LF with heparin was characterized. Pig mucosal heparin and endogenous heparin PG were equally effective in binding LF, whereas heparan sulphate bound with low...Continue Reading

Citations

Apr 29, 2015·Experimental and Molecular Pathology·Michael J WilsonAkhouri A Sinha
Jul 1, 2008·The International Journal of Biochemistry & Cell Biology·Nilana M T BarrosAdriana K Carmona
Nov 20, 2012·Biochimie·Lise BervenAnne Berit C Samuelsen
Nov 9, 2002·Analytical Biochemistry·Ronald I W OsmondDeirdre R Coombe
Oct 22, 2015·PloS One·Lourdes Fernández-DelgadoJavier Monteseirín
Apr 21, 2018·Arteriosclerosis, Thrombosis, and Vascular Biology·Amélie I S SobczakAlan J Stewart
Dec 5, 1998·Journal of Dairy Science·K ShimazakiT Shimo-Oka
Apr 11, 2009·Chembiochem : a European Journal of Chemical Biology·Jonathan F PopplewellDavid G Fernig
Dec 6, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Dominique Legrand
Sep 7, 2001·Journal of Reproductive Immunology·M KrausV Jonáková
Mar 31, 2005·Analytical Biochemistry·Warren C KettDeirdre R Coombe

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