Lactogenic hormone activation of Stat5 and transcription of the beta-casein gene in mammary epithelial cells is independent of p42 ERK2 mitogen-activated protein kinase activity.

The Journal of Biological Chemistry
M WartmannN E Hynes

Abstract

HC11 mammary epithelial cells have been used to characterize molecular events involved in the regulation of milk protein gene expression. Treatment of HC11 cells with the lactogenic hormones prolactin, insulin, and glucocorticoids results in transcription of the beta-casein gene. Prolactin induces a signaling event which involves tyrosine phosphorylation of the mammary gland factor, Stat5, a member of the family of signal transducers and activators of transcription (Stat). Here we show that HC11 cells express two Stat5 proteins, Stat5a and Stat5b. Phosphopeptide and phosphoamino acid analysis of Stat5a and Stat5b immunoprecipitated from phosphate-labeled HC11 cells revealed that both proteins were constitutively phosphorylated on serine. Lactogenic hormone treatment resulted in the appearance of a tyrosine-phosphorylated peptide in both Stat5 proteins. Consistent with this observation, a Western blot analysis of Stat5a and Stat5b showed that lactogenic hormones induced a rapid, transient increase in phosphotyrosine which paralleled the binding of Stat5 to its cognate recognition sequence in the beta-casein gene promoter. Lactogenic hormone treatment of the HC11 cells also led to a rapid activation of the mitogen-activated prote...Continue Reading

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Schmitt-NeyB Groner
Nov 16, 1989·The New England Journal of Medicine·B J DrukerT M Roberts
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K G DanielsonD Medina
Oct 1, 1980·Physiological Reviews·Y J Topper, C S Freeman
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Ruff-JamisonS Cohen
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·X LiuL Hennighausen
Jan 1, 1995·Annual Review of Biochemistry·C Schindler, J E Darnell
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G BoultonG D Yancopoulos
Sep 15, 1995·The Journal of Biological Chemistry·C H StreuliC J Watson
Mar 1, 1995·Trends in Biochemical Sciences·E Cano, L C Mahadevan
May 1, 1994·Trends in Biochemical Sciences·J N IhleO Silvennoinen
Dec 1, 1993·Molecular and Cellular Endocrinology·A R BuckleyM J Blake
Jun 1, 1993·Seminars in Cell Biology·C H Streuli
Oct 1, 1995·Current Opinion in Cell Biology·C D RoskelleyM J Bissell
Apr 5, 1996·The Journal of Biological Chemistry·C H YangL M Pfeffer
Oct 1, 1995·Current Opinion in Genetics & Development·B Groner, F Gouilleux
May 1, 1996·Current Biology : CB·S L Pelech

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Citations

Jan 29, 2000·Journal of Cellular Biochemistry·N CellaN E Hynes
Oct 16, 2009·Functional & Integrative Genomics·Karensa K MenziesKevin R Nicholas
Jan 26, 2008·Journal of Mammary Gland Biology and Neoplasia·Samantha R OakesChristopher J Ormandy
Apr 25, 2003·Biochemical and Biophysical Research Communications·Franklyn F Bolander
Sep 2, 1998·Mechanisms of Ageing and Development·C A La PortaR Comolli
Oct 18, 2001·Molecular and Cellular Endocrinology·C Beisenherz-HussB Groner
Feb 25, 2000·Molecular and Cellular Endocrinology·O GoupilleJ Djiane
Jul 27, 2002·Domestic Animal Endocrinology·Bernd Groner
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·B GronerE Pfitzner
Apr 1, 2000·Cytokine & Growth Factor Reviews·P M GrimleyH Rui
Jan 15, 1998·European Journal of Biochemistry·D Sawka-VerhelleE Van Obberghen
Aug 17, 1999·Annual Review of Nutrition·J M RosenD Hadsell
Apr 11, 2000·Journal of Molecular and Cellular Cardiology·M C RebsamenU Lang
Feb 25, 2005·Vitamins and Hormones·Claudia M LitterstEdith Pfitzner
Sep 8, 2007·Biochemical and Biophysical Research Communications·Amanda M Weaver, Corinne M Silva
Nov 9, 2017·PLoS Genetics·Samantha R OakesChristopher J Ormandy
Jun 13, 2000·Oncogene·T Decker, P Kovarik
Jun 13, 2000·Oncogene·J HerringtonC Carter-Su
Jun 1, 1999·FEBS Letters·S ValgeirsdóttirC H Heldin
May 14, 2014·Molecular Medicine Reports·Cui Feng, Shouqiang Cao
Jun 13, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yong ShaoFeng-Qi Zhao
Feb 23, 2020·In Vitro Cellular & Developmental Biology. Animal·Baosheng WangFeng Zhao
May 23, 1998·The Journal of Biological Chemistry·G M EdwardsC H Streuli
Jun 8, 1999·The Journal of Biological Chemistry·M A OlayioyeN E Hynes

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