Lactogenic hormones alter cellular and extracellular microRNA expression in bovine mammary epithelial cell culture

Journal of Animal Science and Biotechnology
Susumu MuroyaMasaru Nomura

Abstract

Bovine milk contains not only a variety of nutritional ingredients but also microRNAs (miRNAs) that are thought to be secreted by the bovine mammary epithelial cells (BMECs). The objective of this study was to elucidate the production of milk-related miRNAs in BMECs under the influence of lactogenic hormones. According to a microarray result of milk exosomal miRNAs prior to cellular analyses, a total of 257 miRNAs were detected in a Holstein cow milk. Of these, 18 major miRNAs of interest in the milk were selected for an expression analysis in BMEC culture that was treated with or without dexamethasone, insulin, and prolactin (DIP) to induce a lactogenic differentiation. Quantitative polymerase chain reaction (qPCR) results showed that the expressions of miR-21-5p (P = 0.005), miR-26a (P = 0.016), and miR-320a (P = 0.011) were lower in the DIP-treated cells than in the untreated cells. In contrast, the expression of miR-339a (P = 0.017) in the cell culture medium were lower in the DIP-treated culture than in the untreated culture. Intriguingly, the miR-148a expression in cell culture medium was elevated by DIP treatment of BMEC culture (P = 0.018). The medium-to-cell expression ratios of miR-103 (P = 0.025), miR-148a (P < 0.001...Continue Reading

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Citations

Dec 6, 2016·International Journal of Molecular Sciences·Ilaria FlorisIllimar Altosaar
Apr 20, 2017·Pediatric Research·Molly C CarneySteven D Hicks
Jul 19, 2017·International Journal of Molecular Sciences·Duy N DoEveline M Ibeagha-Awemu
Oct 19, 2017·F1000Research·Manoj Kumar Jena
Sep 22, 2017·Diseases·Bodo C Melnik, Gerd Schmitz
Jan 4, 2019·Journal of Translational Medicine·Bodo C Melnik, Gerd Schmitz
Feb 11, 2021·Genes·Elitsa IvanovaFabienne Le Provost
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Gang ZhouJun Zhang
May 13, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shalini JaswalAshok K Mohanty
Dec 10, 2017·Best Practice & Research. Clinical Endocrinology & Metabolism·Bodo C Melnik, Gerd Schmitz
Aug 10, 2021·Journal of Pediatric Gastroenterology and Nutrition·Einat Gutman-IdoRegina Golan-Gerstl

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Methods Mentioned

BETA
electrophoresis
PCR
Assay

Software Mentioned

Affymetrix
STAR
js
Affymetrix® Expression [UNK]
TargetScan
DAVID

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