Lactose metabolism and cellulase production in Hypocrea jecorina: the gal7 gene, encoding galactose-1-phosphate uridylyltransferase, is essential for growth on galactose but not for cellulase induction

Molecular Genetics and Genomics : MGG
Bernhard SeibothC P Kubicek

Abstract

Lactose is at present the only soluble carbon source which can be used economically for the production by Hypocrea jecorina (= Trichoderma reesei) of cellulases or heterologous proteins under the control of cellulase expression signals. However, the mechanism by which lactose triggers the formation of cellulases is unknown. To enhance our understanding of lactose metabolism and its relationship to cellulase formation, we have cloned and characterized the gal7 gene (for galactose-1-phosphate uridylyltransferase) of H. jecorina. The gene encodes a polypeptide of 43.8 kDa, the sequence of which exhibits a moderate level of identity (about 50%) to that of the Gal7 proteins of Saccharomyces cerevisiae and Kluyveromyces lactis, and contains an active-site signature typical for galactose-1-phosphate uridylyltransferase family 1. H. jecorina gal7 is not clustered with other genes of galactose metabolism. A single 1.7-kb transcript is synthesized constitutively during the rapid growth phase and accumulated to twice this level during incubation in the presence of D-galactose and L-arabinose and the corresponding polyols (dulcitol, arabitol). A gal7 deletion mutant, constructed by replacing the gal7 reading frame by the H. jecorina pyr4 g...Continue Reading

Citations

Nov 15, 2011·Applied Microbiology and Biotechnology·Fatma UzbasBernhard Seiboth
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Erzsébet FeketeLevente Karaffa
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A ZnameroskiN Louise Glass
May 7, 2004·European Journal of Biochemistry·Manuela PailChristian P Kubicek
Aug 9, 2011·Applied and Environmental Microbiology·Ulla ChristensenRonald P de Vries
Feb 5, 2005·Applied and Environmental Microbiology·Bernhard SeibothChristian P Kubicek
Sep 3, 2009·Biotechnology for Biofuels·Christian P KubicekBernhard Seiboth
Jan 13, 2006·Applied Microbiology and Biotechnology·Nicole Teixeira SehnemAldo J P Dillon
Nov 27, 2015·Biotechnology for Biofuels·Zbigniew LazarJean-Marc Nicaud
May 9, 2006·Computational Biology and Chemistry·Ozlem Demir, Isil Aksan Kurnaz
Aug 17, 2005·FEMS Microbiology Reviews·Nina AroMerja Penttilä
Aug 5, 2003·Acta Microbiologica Et Immunologica Hungarica·Monika Schmoll, C P Kubicek
Feb 14, 2012·FEMS Microbiology Letters·Erzsébet FeketeLevente Karaffa
Apr 20, 2006·Microbiology·Levente KaraffaBernhard Seiboth

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