Lactose synthesis in a monotreme, the echidna (Tachyglossus aculeatus): isolation and amino acid sequence of echidna alpha-lactalbumin

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M MesserD C Shaw

Abstract

alpha-Lactalbumin and lysozyme were each isolated from echidna (Tachyglossus aculeatus) milk by gel permeation and ion exchange chromatography. The alpha-lactalbumin modified the action of echidna milk galactosyltransferase to promote the synthesis of lactose but had very little effect on bovine galactosyltransferase. Echidna alpha-lactalbumin is a glycosylated protein with an apparent molecular weight of 20,000 (SDS-PAGE) whose concentration in the milk is very low compared with the concentrations of alpha-lactalbumin in the milk of other species. Its amino acid sequence is more similar to that of another monotreme, the platypus (Ornithorhynchus anatinus), than to the sequences of eutherian or marsupial alpha-lactalbumins. Echidna milk lysozyme, even at high concentrations, did not promote the synthesis of lactose by either echidna or bovine galactosyltransferase. We conclude that lactose synthesis in the echidna occurs by the same mechanism as that found in the platypus and other mammals.

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Citations

Dec 12, 2003·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Peggy D Rismiller, Michael W McKelvey
May 25, 2004·Antimicrobial Agents and Chemotherapy·Delphine GirlichPatrice Nordmann
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Mar 23, 2012·Animal : an International Journal of Animal Bioscience·T UrashimaM Messer

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