Lactulose and mannitol intestinal permeability detected by capillary electrophoresis

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Rita ParoniEmanuele Bosi

Abstract

Aim of this study was to set up a method by capillary electrophoresis to detect lactulose and mannitol in urine after an oral load, and to estimate the intestinal permeability in controls and in type I diabetes patients. The underivatized carbohydrates were monitored by indirect UV detection using sorbate, cetyltrimethylammonium bromide and LiOH as background electrolyte. Urines were purified by solid phase extraction, shaken with cation exchange resin, filtered and analysed. Carbohydrates migrated in <10 min in relation to their pK(a) and M(r). Controls (n = 33) and patients (n = 23) had an excretion ratio lactulose/mannitol 0.025 (0.018-0.051) and 0.067 (0.050-0.127), respectively (p < 0.01, median, interquartile range).

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Citations

Sep 19, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Paweł KubicaPiotr Landowski
Jan 30, 2007·Journal of Chromatography. a·M L Sanz, I Martínez-Castro
Aug 23, 2011·Biotechnology Advances·Parmjit S Panesar, Shweta Kumari
Apr 29, 2008·Clinical Biochemistry·Alfonso M LostiaMaurizio Simmaco
Aug 11, 2017·Nutritional Neuroscience·Barbara De SantisMaria Elisabetta Raggi
Aug 14, 2019·Carbohydrate Research·Jiří Pazourek
Jun 29, 2021·Journal of Autoimmunity·Mia Øgaard MønstedMartin Haupt-Jorgensen
Nov 4, 2021·Cellular and Molecular Life Sciences : CMLS·Hanna Ilchmann-DiounouSandrine Menard

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