LAESI mass spectrometry imaging as a tool to differentiate the root metabolome of native and range-expanding plant species

Planta
Purva KulkarniPaolina Garbeva

Abstract

LAESI-MSI, an innovative high-throughput technique holds a unique potential for untargeted detection, profiling and spatial localization of metabolites from intact plant samples without need for extraction or extensive sample preparation. Our understanding of chemical diversity in biological samples has greatly improved through recent advances in mass spectrometry (MS). MS-based-imaging (MSI) techniques have further enhanced this by providing spatial information on the distribution of metabolites and their relative abundance. This study aims to employ laser-ablation electrospray ionization (LAESI) MSI as a tool to profile and compare the root metabolome of two pairs of native and range-expanding plant species. It has been proposed that successful range-expanding plant species, like introduced exotic invaders, have a novel, or a more diverse secondary chemistry. Although some tests have been made using aboveground plant materials, tests using root materials are rare. We tested the hypothesis that range-expanding plants possess more diverse root chemistries than native plant species. To examine the root chemistry of the selected plant species, LAESI-MSI was performed in positive ion mode and data were acquired in a mass range of ...Continue Reading

References

Mar 29, 2002·Nature·Gian-Reto WaltherFranz Bairlein
Mar 18, 2006·Science·R Graham CooksJustin M Wiseman
Dec 7, 2006·Biology Letters·Naomi Cappuccino, J Thor Arnason
Jun 4, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Zhaoyang Chen, Akos Vertes
Jun 15, 2011·Ecology·Urs SchaffnerRagan M Callaway
Jul 17, 2012·Bioinformatics·Sebastian Gibb, Korbinian Strimmer
Sep 22, 2012·Mass Spectrometry Reviews·Chunping WuDemian R Ifa
Jan 24, 2014·Natural Product Reports·Nanna BjarnholtChristian Janfelt
Sep 2, 2014·BMC Bioinformatics·Philippe BardouChristophe Klopp
Apr 22, 2015·Nucleic Acids Research·Jianguo XiaDavid S Wishart
Sep 1, 2016·Journal of Chemical Ecology·John K SeniorJulianne M O'Reilly-Wapstra
Jan 14, 2017·Current Opinion in Chemical Biology·Daniel PetrasPieter C Dorrestein

❮ Previous
Next ❯

Citations

Dec 2, 2020·International Journal of Molecular Sciences·Leonardo Perez de SouzaAlisdair Fernie
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Jul 3, 2021·Metabolites·Muhammad Syamsu RizaludinPaolina Garbeva

❮ Previous
Next ❯

Methods Mentioned

BETA
metabolomics profiling
PCA

Software Mentioned

Metaboanalyst
LAESI
ProteaPlot
R scripts
MALDIquant R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.