Lagerstroemia speciosa (L.) Pers. triggers oxidative stress mediated apoptosis via intrinsic mitochondrial pathway in HepG2 cells

Environmental Toxicology
Rohit Singh Thakur, Devaraj Ezhilarasan

Abstract

Hepatocellular carcinoma is the second leading cause of cancer-related mortality worldwide. Lagerstroemia speciosa Pers. (Lythraceae) commonly known as Banaba has been used in different forms in traditional medicinal systems for treating various diseases which include diabetes and obesity. In this study, we investigated the cytotoxic potential of ethanolic Banaba leaf extract (EBLE) in HepG2 cells. The phytochemical analysis of EBLE was performed by HPTLC. HepG2 cells were treated with EBLE at 25, 50, 100, and 150 μg/mL concentrations, and cytotoxicity was evaluated by MTT assay. Oxidative stress was assessed by the evaluation of lipid peroxidation, superoxide dismutase, and reduced glutathione. Apoptosis-related morphology was investigated by acridine orange and ethidium bromide (AO/EB) dual staining. Mitochondrial membrane potential (ΔΨm) was evaluated by JC-1 staining. Apoptosis-related marker genes were evaluated by qPCR. HPTLC analysis confirmed the presence of corosolic acid (12.87 μg/mg), berberine (3.19 μg/mg), and gallic acid (2.94 μg/mg) in EBLE. EBLE treatments caused significant and concentration-dependent cytotoxicity and oxidative stress in HepG2 cells. Dual staining with AO/EB confirmed membrane distortion and nu...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Feb 1, 1984·Analytical Biochemistry·E F Roth, H S Gilbert
May 25, 2007·Cancer Biomarkers : Section a of Disease Markers·Michael L VolkJorge A Marrero
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Yan Li, Robert C G Martin
Nov 19, 2011·Phytotherapy Research : PTR·Sidney J StohsGilbert R Kaats
Mar 9, 2012·Advances in Experimental Medicine and Biology·Jérome EstaquierBernard Mignotte
Jan 1, 2006·CSH Protocols·Shailaja KasibhatlaDouglas R Green
Jan 16, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lu ZhengJing Li
Jun 6, 2014·Annals of Medicine·Miriam MartiniEmilio Hirsch
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Apr 2, 2015·Expert Review of Gastroenterology & Hepatology·Michael C WallaceLeon A Adams
Sep 13, 2015·Apoptosis : an International Journal on Programmed Cell Death·Hala Gali-MuhtasibNadine Darwiche
Oct 21, 2015·Frontiers in Plant Science·Marcelo FridlenderHinanit Koltai
Jun 18, 2016·Surgical Oncology·Miral Sadaria GrandhiTimothy M Pawlik
Sep 8, 2016·Molecular Medicine Reports·Hai-Yan WangYu-Lan Zheng
Jan 9, 2017·Progress in Retinal and Eye Research·Margaret E MaesRobert W Nickells
Jan 20, 2017·BMC Complementary and Alternative Medicine·Bipransh Kumar TiwaryRanadhir Chakraborty
Jun 3, 2017·Scientific Reports·Mingzhen ZhangBuyong Ma
Dec 1, 2017·Journal of Advanced Pharmaceutical Technology & Research·Thangavelu LakshmiRamasamy Rajendran
Dec 5, 2017·Trends in Cancer·Peter J Burke
Dec 6, 2017·Oxidative Medicine and Cellular Longevity·María José VallejoMarcelo Grijalva
Jun 2, 2018·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Devaraj Ezhilarasan
Nov 20, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Devaraj EzhilarasanNandhigam Ashok Vardhan
Feb 25, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Muhammad ImranMohammad S Mubarak
Mar 30, 2019·Human & Experimental Toxicology·S Gheena, D Ezhilarasan

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