PMID: 9422596Jan 9, 1998Paper

Lagging-strand replication from the ssoA origin of plasmid pMV158 in Streptococcus pneumoniae: in vivo and in vitro influences of mutations in two conserved ssoA regions

Journal of Bacteriology
M G KramerM Espinosa

Abstract

The streptococcal plasmid pMV158 replicates by the rolling-circle mechanism. One feature of this replication mechanism is the generation of single-stranded DNA intermediates which are converted to double-stranded molecules. Lagging-strand synthesis initiates from the plasmid single-stranded origin, sso. We have used the pMV158-derivative plasmid pLS1 (containing the ssoA type of lagging-strand origin) and a set of pLS1 derivatives with mutations in two conserved regions of the ssoA (the recombination site B [RS(B)] and a conserved 6-nucleotide sequence [CS-6]) to identify sequences important for plasmid lagging-strand replication in Streptococcus pneumoniae. Cells containing plasmids with mutations in the RS(B) accumulated 30-fold more single-stranded DNA than cells containing plasmids with mutations in the CS-6 sequence. Specificity of lagging-strand synthesis was tested by the development of a new in vitro replication system with pneumococcal cell extracts. Four major initiation sites of lagging-strand DNA synthesis were observed. The specificity of initiation was maintained in plasmids with mutations in the CS-6 region. Mutations in the RS(B) region, on the other hand, resulted in the loss of specific initiation of lagging-s...Continue Reading

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Citations

Mar 29, 2001·Journal of Bacteriology·L R BarranE S Bromfield
Nov 26, 2008·Journal of Bacteriology·Fabián Lorenzo-Díaz, Manuel Espinosa
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·David BikardDidier Mazel
Aug 21, 2012·FEMS Microbiology Letters·Lívia FecskeováPeter Pristaš
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G KramerS A Khan
Oct 13, 2007·Biotechnology Letters·G A Somkuti, D H Steinberg
Mar 28, 2012·FEMS Microbiology Letters·Wilfried RozhonBrigitte Poppenberger
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·G del SolarR Díaz-Orejas
Jun 25, 2015·Microbiology Spectrum·J A Ruiz-MasóG Del Solar

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