PMID: 3770980Sep 1, 1986Paper

Lagtime: a program for calculating coupled enzyme assay parameters

International Journal of Bio-medical Computing
S P Brooks, C H Suelter

Abstract

A program is described for calculating either (i) the time required for the observed rate to approximate the rate of the enzyme under study (the lagtime) when the concentrations of the auxiliary enzymes are known or (ii) the units of auxiliary enzymes needed to obtain a desired lagtime. The calculations for these coupled enzyme systems also apply to cases where one of the intermediates undergoes mutarotation; for example, equations for coupled reactions involving two enantiomers of glucose as intermediates incorporate the rate constants for mutarotation. When two auxiliary enzymes are used, the program also minimizes the total cost of the assay if the price per unit of the coupling enzymes are known. The equations used are those of S.P.J. Brooks et al. (Can. J. Biochem. Cell Biol. 62 (1984) 945-955; 956-963.

References

Oct 15, 1979·Analytical Biochemistry·W W Cleland
Jul 1, 1969·Biochemistry·W R McClure
Feb 15, 1973·Biochimica Et Biophysica Acta·J S Easterby
Oct 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·S P BrooksC H Suelter
Oct 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·S P BrooksC H Suelter
Oct 1, 1981·The Biochemical Journal·J S Easterby

❮ Previous
Next ❯

Citations

Aug 3, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ryan A V Bell, Kenneth B Storey
Jan 1, 1989·Analytical Biochemistry·S P Brooks, C H Suelter
Apr 1, 1988·Journal of Molecular Recognition : JMR·S P BrooksC H Suelter
Oct 6, 2018·Bulletin of Mathematical Biology·Justin Eilertsen, Santiago Schnell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.