LAHEDES: the LAGLIDADG homing endonuclease database and engineering server.

Nucleic Acids Research
Gregory K TaylorBarry L Stoddard

Abstract

LAGLIDADG homing endonucleases (LHEs) are DNA cleaving enzymes, also termed 'meganucleases' that are employed as gene-targeting reagents. This use of LHEs requires that their DNA specificity be altered to match sequences in genomic targets. The choice of the most appropriate LHE to target a particular gene is facilitated by the growing number of such enzymes with well-characterized activities and structures. 'LAHEDES' (The LAGLIDADG Homing Endonuclease Database and Engineering Server) provides both an online archive of LHEs with validated DNA cleavage specificities and DNA-binding interactions, as well as a tool for the identification of DNA sequences that might be targeted by various LHEs. Searches can be performed using four separate scoring algorithms and user-defined choices of LHE scaffolds. The webserver subsequently provides information regarding clusters of amino acids that should be interrogated during engineering and selection experiments. The webserver is fully open access and can be found at http://homingendonuclease.net.

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Citations

Apr 23, 2013·Trends in Biotechnology·Nancy PodevinJosep M Casacuberta
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Nov 19, 2013·PloS One·Zhengxing ZhangYi Sheng
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Sep 11, 2020·ACS Synthetic Biology·Shingo SuzukiTakanori Miki
Oct 12, 2021·Transgenic Research·Benoit DaniloMarianne Mazier

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Methods Mentioned

BETA
gene modification
transgenic
targeted
flow cytometry

Software Mentioned

PWM
LAGLIDADG Homing Endonuclease Database and Engineering Server ...
HomeBase webserver
Endonuclease Browser
LAHEDES
Module Search
Genomic Search

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