LambdaZLG6: a phage lambda vector for high-efficiency cloning and surface expression of cDNA libraries on filamentous phage

Gene
L S JespersP E Stanssens

Abstract

We describe a vector, lambdaZLG6, combining the high efficiency of cDNA library cloning in bacteriophage lambda with filamentous phage display of cDNA-encoded products. The cDNAs are expressed as fusions to the 3' end of M13 gene VI. The lambdaZLG library is converted to a pZLG6-cDNA phagemid library by in vivo mass excision. Helper phage infection generates a library of phagemid particles displaying the cDNA-encoded products and containing the corresponding nucleotide sequences within.

Citations

May 29, 2004·Pharmacogenomics·Nikolai KleySebastian Meier-Ewert
Oct 8, 2004·Journal of Biochemistry and Molecular Biology·Ha-Tan KangYeon Gyu Yu
Apr 1, 2010·Expert Opinion on Drug Discovery·Yoichi TakakusagiKengo Sakaguchi
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Feb 19, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuliya Georgieva, Zoltán Konthur
Apr 15, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elisa Beghetto, Nicola Gargano
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Mar 10, 2021·Current Microbiology·Taruna AnandBhupendra N Tripathi
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