Lamellar granule secretion starts before the establishment of tight junction barrier for paracellular tracers in mammalian epidermis.

PloS One
Akemi Ishida-YamamotoHajime Iizuka

Abstract

Defects in epidermal barrier function and/or vesicular transport underlie severe skin diseases including ichthyosis and atopic dermatitis. Tight junctions (TJs) form a single layered network in simple epithelia. TJs are important for both barrier functions and vesicular transport. Epidermis is stratified epithelia and lamellar granules (LGs) are secreted from the stratum granulosum (SG) in a sequential manner. Previously, continuous TJs and paracellular permeability barriers were found in the second layer (SG2) of SG in mice, but their fate and correlation with LG secretion have been poorly understood. We studied epidermal TJ-related structures in humans and in mice and found occludin/ZO-1 immunoreactive multilayered networks spanning the first layer of SG (SG1) and SG2. Paracellular penetration tracer passed through some TJs in SG2, but not in SG1. LG secretion into the paracellular tracer positive spaces started below the level of TJs of SG1. Our study suggests that LG-secretion starts before the establishment of TJ barrier in the mammalian epidermis.

References

Apr 1, 1975·The Journal of Cell Biology·P M Elias, D S Friend
Jul 1, 1971·The Journal of Investigative Dermatology·K Hashimoto
Jun 10, 1998·The Journal of Investigative Dermatology·K MoritaS Tsukita
Jun 18, 2002·European Journal of Cell Biology·Johanna M BrandnerIngrid Moll
May 14, 2004·The Journal of Investigative Dermatology·Akemi Ishida-YamamotoHajime Iizuka
Sep 9, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Johanna M Brandner
Aug 8, 2009·The American Journal of Pathology·Nina KirschnerJohanna M Brandner
Dec 10, 2009·The Journal of Experimental Medicine·Akiharu KuboMasayuki Amagai
Jun 1, 1983·The Journal of Investigative Dermatology·P M Elias
Aug 25, 2010·European Journal of Cell Biology·Nina KirschnerJohanna M Brandner
Oct 20, 2010·Experimental Dermatology·Satomi IgawaAkemi Ishida-Yamamoto
Dec 17, 2010·The Journal of Investigative Dermatology·Takuo YukiShintaro Inoue
Dec 18, 2010·The Journal of Allergy and Clinical Immunology·Anna De BenedettoLisa A Beck
Dec 31, 2010·The Journal of Investigative Dermatology·Nina KirschnerJohanna M Brandner
Feb 25, 2011·American Journal of Physiology. Cell Physiology·Yanfang GuanMarshall H Montrose

❮ Previous
Next ❯

Citations

May 29, 2013·Journal of Dermatological Science·Kazue YoshidaAkiharu Kubo
Jan 21, 2016·European Journal of Cell Biology·Christiane MathesClaus-Michael Lehr
Jan 29, 2013·Journal of Dermatological Science·Kazumitsu Sugiura
Jan 7, 2014·The Journal of Investigative Dermatology·Masashi MiyaiToshihiko Hibino
Sep 1, 2015·The American Journal of Pathology·Robert GruberJohanna M Brandner
Apr 4, 2015·Tissue Barriers·J M BrandnerA De Benedetto
Nov 20, 2014·Cell and Tissue Research·Akemi Ishida-Yamamoto, Satomi Igawa
Feb 11, 2015·Cell and Tissue Research·Johanna M Brandner, Jörg D Schulzke
Jan 20, 2018·The Journal of Dermatology·Akemi Ishida-YamamotoMasaru Honma
Apr 29, 2018·Experimental Dermatology·Akemi Ishida-YamamotoMari Kishibe
Jul 19, 2018·Experimental Dermatology·Mariko Yokouchi, Akiharu Kubo
Nov 18, 2016·Pflügers Archiv : European journal of physiology·Katja Bäsler, Johanna M Brandner
Feb 8, 2020·Scientific Reports·Sophia BergmannJohanna M Brandner
Oct 18, 2020·International Journal of Molecular Sciences·Hai Le Thanh NguyenFrançois Niyonsaba
May 25, 2021·Journal of Tissue Engineering and Regenerative Medicine·Masato MurakamiMitsuru Akashi

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
biopsy
electron microscopy

Software Mentioned

Olympus FV1000
Adobe Photoshop CS4

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.