Lamin B phosphorylation by protein kinase calpha and proteolysis during apoptosis in human leukemia HL60 cells.

The Journal of Biological Chemistry
T ShimizuY Pommier

Abstract

Protein phosphorylation plays an important role in signal transduction, but its involvement in apoptosis still remains unclear. In this report, the p53-null human leukemia HL60 cells were used to investigate phosphorylation and degradation of lamin B during apoptosis. We found that lamin B was phosphorylated within 1 h after addition of the DNA topoisomerase I inhibitor, camptothecin, and that lamin B phosphorylation preceded lamin B degradation and DNA fragmentation. Using a cell-free system we also found that cytosol from camptothecin-treated cells induced lamin B phosphorylation and degradation in isolated nuclei from untreated HL60 cells. Lamin B phosphorylation was prevented by the protein kinase C (PKC) inhibitor 7-hydroxystaurosporine (UCN-01) but not by the Cdc2 inhibitor, flavopiridol. Phosphorylation of lamin B was inhibited by immunodepletion of PKCalpha from activated cytosol and was restored by addition of purified PKCalpha. PKCalpha activity also increased rapidly as lamin B was phosphorylated after initiation of the apoptotic response in HL60 cells. These data suggest that lamin B is phosphorylated by PKCalpha and proteolyzed before DNA fragmentation in HL60 cells undergoing apoptosis.

References

Aug 1, 1992·Seminars in Cell Biology·E A Nigg
Jan 1, 1988·Annual Review of Cell Biology·L Gerace, B Burke
Dec 1, 1987·The Journal of Antibiotics·I TakahashiH Nakano
Nov 9, 1995·Biochimica Et Biophysica Acta·B T Gjertsen, S O Døskeland
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y A LazebnikW C Earnshaw
Jun 1, 1995·Current Opinion in Cell Biology·W C Earnshaw
Feb 25, 1994·Science·L ShiA H Greenberg
Apr 1, 1994·Experimental Cell Research·R BertrandY Pommier
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·W F De AzevedoS H Kim
May 1, 1996·Current Biology : CB·A M Chinnaiyan, V M Dixit
Sep 15, 1996·Experimental Cell Research·R G ShaoY Pommier
Oct 18, 1996·Cell·E S AlnemriJ Yuan
Dec 1, 1996·The Journal of Cell Biology·L RaoE White
Apr 7, 1997·Biochemical and Biophysical Research Communications·B ZhivotovskyS Orrenius
Aug 22, 1997·The Journal of Biological Chemistry·P CollasJ C Courvalin
Jul 1, 1996·Trends in Cell Biology·M Whyte

❮ Previous
Next ❯

Citations

Jul 12, 2002·Journal of Cellular Biochemistry·Alberto M MartelliLucio Cocco
Aug 14, 2001·Journal of Cellular Biochemistry·A M MartelliR Bortul
Mar 12, 2013·Chromosoma·Dan N Simon, Katherine L Wilson
Feb 16, 2005·Apoptosis : an International Journal on Programmed Cell Death·B ZhangR G Fenton
Feb 24, 2010·Journal of Molecular Histology·Juan Pablo PetitiAlicia Inés Torres
Nov 18, 2000·Cellular Signalling·W J van Blitterswijk, B Houssa
Jul 24, 2002·Cellular Signalling·Giovanna TabelliniAlberto M Martelli
Apr 6, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·L OrlandiN Zaffaroni
Apr 20, 2001·Critical Reviews in Oncology/hematology·H H Sedlacek
Mar 11, 2011·Molecular Biology of the Cell·Kuei-Hua ChangKavita Shah
Mar 23, 2005·Acta Pharmacologica Sinica·Qing-hui MengSai-jun Fan
Nov 23, 2013·PloS One·Nelly GodefroyAndréa C LeBlanc
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·D ZhangJ Lieberman
Jan 20, 2005·The Journal of Cell Biology·Daniel R CroftMichael F Olson
Jun 4, 2008·The Journal of Cell Biology·Panagiota PanagopoulouYassemi Capetanaki
Jul 21, 2001·Cell Death and Differentiation·T SandalS O Døskeland
Dec 29, 2007·Endocrinology·Miriam Horovitz-FriedSanford R Sampson
May 24, 2000·Oncogene·T CrossJ M Lord
Oct 21, 2004·American Journal of Hematology·Paul CoppoKaiss Lassoued
Oct 9, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Apurva K SrivastavaRalph E Parchment
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Tanaya Roychowdhury, Samit Chattopadhyay
Feb 23, 2021·The Journal of Biological Chemistry·An-Angela N VanAlexandra C Newton
May 12, 2000·Journal of Structural Biology·Y GruenbaumM Cohen
Dec 14, 2002·Biochemical and Biophysical Research Communications·Do Hoon KimWoo Keun Song
Dec 31, 2002·Biochemical and Biophysical Research Communications·Germán GallardoLuisa F Fanjul
Oct 3, 1999·Archives of Pathology & Laboratory Medicine·X ZhangF Kiechle

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Biochemical and Biophysical Research Communications
B ZhivotovskyS Orrenius
The International Journal of Biochemistry & Cell Biology
M P de MoelJ J De Pont
Current Medicinal Chemistry. Anti-cancer Agents
Rita NahtaFrancisco J Esteva
© 2022 Meta ULC. All rights reserved