Lamin B1 mapping reveals the existence of dynamic and functional euchromatin lamin B1 domains.

Nature Communications
Laura Pascual-ReguantSandra Peiró

Abstract

Lamins (A/C and B) are major constituents of the nuclear lamina (NL). Structurally conserved lamina-associated domains (LADs) are formed by genomic regions that contact the NL. Lamins are also found in the nucleoplasm, with a yet unknown function. Here we map the genome-wide localization of lamin B1 in an euchromatin-enriched fraction of the mouse genome and follow its dynamics during the epithelial-to-mesenchymal transition (EMT). Lamin B1 associates with actively expressed and open euchromatin regions, forming dynamic euchromatin lamin B1-associated domains (eLADs) of about 0.3 Mb. Hi-C data link eLADs to the 3D organization of the mouse genome during EMT and correlate lamin B1 enrichment at topologically associating domain (TAD) borders with increased border strength. Having reduced levels of lamin B1 alters the EMT transcriptional signature and compromises the acquisition of mesenchymal traits. Thus, during EMT, the process of genome reorganization in mouse involves dynamic changes in eLADs.

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Citations

Jan 22, 2019·Nucleus·Frida ForsbergPhilippe Collas
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Datasets Mentioned

BETA
GSE96033

Methods Mentioned

BETA
DamID
immunoprecipitation
confocal microscopy
ChIP-seq
RNA-seq
PCR
Hi-C
fluorescence recovery after photobleaching
the
ChIP

Software Mentioned

DamID
ImageJ
UCSC Genome Browser
Enrichr
AF

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