Laminar analysis of initiation and spread of epileptiform discharges in three in vitro models

Brain Research Bulletin
Sándor BorbélyIldikó Világi

Abstract

Overexcitation of neuronal networks in some forebrain structures and pathological synchronization of neuronal activity play crucial role in epileptic seizures. Seizure activity can be elicited experimentally by different convulsants. Because of various distribution of excitatory and inhibitory connections in the neocortex there might be laminar differences in seizure sensitivity. Current source density (CSD) analysis or immunocytochemical c-Fos localization offer suitable tools to localize increased activation of neurons during seizure. In the present experiments, interictal epileptiform activity elicited by 4-aminopiridine, bicuculline or Mg(2+)-free solution was recorded with a 16-channel multielectrode assembly in different layers of the somatosensory cortex, and CSDs were calculated. Parallel c-Fos immunocytochemistry was applied. Each convulsant elicited characteristic activation pattern. 4-aminopiridine induced relatively short discharges, which were associated with a huge sink in layer V, the sink and source pattern was relatively simple. Mg(2+)-free solution elicited the longest discharges, sinks appeared typically in the supragranular layers II and III than quickly distributed toward layers V and VI. Bicuculline induce...Continue Reading

References

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Citations

Oct 6, 2015·Journal of Neuroscience Methods·Roland S G JonesMark O Cunningham
Oct 15, 2013·The European Journal of Neuroscience·Sándor BorbélyIldikó Világi
Jun 11, 2019·Brain Connectivity·Veronika BaloghSándor Borbély
Apr 7, 2012·Physiological Research·P MarešH Kubová
Jun 29, 2017·Cell Reports·Michael WenzelRafael Yuste

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