Laminar Flow Protects Vascular Endothelial Tight Junctions and Barrier Function via Maintaining the Expression of Long Non-coding RNA MALAT1

Frontiers in Bioengineering and Biotechnology
Fangfang YangJing Zhou

Abstract

Atherosclerotic plaque preferentially develops in arterial curvatures and branching regions, where endothelial cells constantly experience disturbed blood flow. By contrast, the straight arteries are generally protected from plaque formation due to exposure of endothelial cells to vaso-protective laminar blood flow. However, the role of flow patterns on endothelial barrier function remains largely unclear. This study aimed to investigate new mechanisms underlying the blood flow pattern-regulated endothelial integrity. Exposure of human endothelial cells to pulsatile shear (PS, mimicking the laminar flow) compared to oscillatory shear (OS, mimicking the disturbed flow) increased the expressions of long non-coding RNA MALAT1 and tight junction proteins ZO1 and Occludin. This increase was abolished by knocking down MALAT1 or Nesprin1 and 2. PS promoted the association between Nesprin1 and SUN2 at the nuclear envelopes, and induced a nuclear translocation of β-catenin, likely through enhancing the interaction between β-catenin and Nesprin1. In the in vivo study, mice were treated via intraperitoneal injection with β-catenin agonist SKL2001 or its inhibitor XAV939, and they were then subjected to Evans blue injection to assess aorti...Continue Reading

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Citations

Sep 20, 2020·Bioengineering·Vanessa AllwardtVirginia Pensabene
May 6, 2021·APL Bioengineering·Rossana RautiBen M Maoz
Jun 22, 2021·Antioxidants & Redox Signaling·Minchun JiangLi Wang

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Methods Mentioned

BETA
nuclear translocation
PCR
fluorescence microscopy
Assay
pull down
dissection
Fluorescence
transfection
co-immunoprecipitation assay
immunoprecipitation assay

Software Mentioned

Image
Pro Plus

Related Concepts

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Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

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