Laminar specific alterations of thalamocortical projections in organotypic cultures following layer 4 disruption in ferret somatosensory cortex

The European Journal of Neuroscience
S L PalmerS L Juliano

Abstract

The developing neocortex influences the growth of thalamocortical projections. Layer 4 in particular receives the majority of input from the thalamus and is important in instructing thalamic afferents to terminate. Previous in vivo experiments demonstrated that disruption of layer 4 during corticogenesis in ferret somatosensory cortex by application of methylazoxy methanol acetate (MAM) prevents proper termination of thalamic afferents in appropriate cortical regions. To further explore the role of layer 4 in thalamocortical development, we prepared organotypic cocultures consisting of normal gestational day 0 (P0) ferret thalamus paired with normal, embryonic day 33 (E33), or E38 MAM-treated cortex obtained from ferrets at either P0 or P7. Injection of MAM on E33 disrupts layer 4 formation, whereas similar injections on E38 interfere with layer 2 formation. The cocultures grew together for a number of days, then discrete injections of either fluorescent dextrans or 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI) were made into the thalamic piece. The labeled thalamic afferents that grew into the cortical slice were analysed and the sites of their terminations quantified after 3, 5, or 7-10 days in cul...Continue Reading

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Citations

Feb 4, 2006·Acta Neuropathologica·Volney L SheenChristopher A Walsh
Jun 5, 2003·Brain Research Bulletin·Diego Garbossa, Alessandro Vercelli
Apr 20, 2007·Cerebral Cortex·Sylvie PoluchSharon L Juliano
Dec 7, 2007·Experimental Neurology·Alisa W Schaefer, Sharon L Juliano
Feb 12, 2008·Developmental Neurobiology·Alisa SchaeferSharon Juliano
Aug 14, 2008·Behavioral and Brain Functions : BBF·Claudia PatrignaniPaola F Zaratin
Mar 18, 2005·Cerebral Cortex·Debra F McLaughlin, Sharon L Juliano
Apr 26, 2008·Synapse·Abigail Snyder-KellerPatricio O'Donnell
May 18, 2021·Frontiers in Cell and Developmental Biology·Carlotta Gilardi, Nereo Kalebic

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