Laminin-8/9 is synthesized by rat glomerular mesangial cells and is required for PDGF-induced mesangial cell migration

Kidney International
Kim M Hansen, Christine K Abrass

Abstract

Laminin (LM), the major glycoprotein component of basement membranes is expressed as multiple isoforms in a developmentally regulated and tissue-specific manner. LM alpha4 has a limited tissue distribution and is highly expressed in the developing glomerulus. In the present study, we investigate the in vivo and in vitro expression and function of LM alpha4 in the glomerulus. LM alpha4 expression was examined by Northern blot, reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunofluorescence microscopy. Mesangial cells (MC) were plated on purified LM-1, LM-2, and LM-8/9. Immunofluorescence microscopy was performed to examine the cellular phenotypes induced by LM-1 and LM-8/9. A modified Boyden chamber method was used to assess laminin participation in platelet-derived growth factor (PDGF)-stimulated migration. mRNA for LMalpha4 is expressed in cultured rat MC, and isolated rat and mouse glomeruli, but not in cultured rat glomerular epithelial cells or glomerular endothelial cells. Using antibodies specific for LM alpha4, a 240 kD band was detected in MC extract and a slightly smaller band was identified in extracted rat glomeruli. Purified LM-8/9 had MC adhesive activity comparable to LM-1 and LM-2. ...Continue Reading

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Citations

Jun 6, 2003·Kidney International·Dale R Abrahamson
May 13, 2006·The Journal of Biological Chemistry·Sujata Lakhe-ReddyJeffrey R Schelling
Jun 19, 2013·Biomaterials·David D R SebingerCarsten Werner
Jun 22, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Taichi IshikawaManuel Patarroyo
May 5, 2006·American Journal of Physiology. Cell Physiology·Anne K BerfieldChristine K Abrass
Aug 13, 2020·Signal Transduction and Targeted Therapy·Zhaoqing WangXiyun Yan

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