laminin alpha 1 gene is essential for normal lens development in zebrafish

BMC Developmental Biology
Natalya S ZinkevichElena V Semina

Abstract

Laminins represent major components of basement membranes and play various roles in embryonic and adult tissues. The functional laminin molecule consists of three chains, alpha, beta and gamma, encoded by separate genes. There are twelve different laminin genes identified in mammals to date that are highly homologous in their sequence but different in their tissue distribution. The laminin alpha -1 gene was shown to have the most restricted expression pattern with strong expression in ocular structures, particularly in the developing and mature lens. We identified the zebrafish lama1 gene encoding a 3075-amino acid protein (lama1) that possesses strong identity with the human LAMA1. Zebrafish lama1 transcripts were detected at all stages of embryo development with the highest levels of expression in the developing lens, somites, nervous and urogenital systems. Translation of the lama1 gene was inhibited using two non-overlapping morpholino oligomers that were complementary to sequences surrounding translation initiation. Morphant embryos exhibited an arrest in lens development and abnormalities in the body axis length and curvature. These results underline the importance of the laminin alpha 1 for normal ocular development and ...Continue Reading

References

Jun 1, 1991·Current Eye Research·C M Parmigiani, J W McAvoy
Dec 1, 1986·Ophthalmic Paediatrics and Genetics·J S Green, G J Johnson
Mar 1, 1968·The Journal of Heredity·D S Varnum, L C Stevens
Jan 1, 1984·Differentiation; Research in Biological Diversity·C Parmigiani, J McAvoy
Apr 1, 1980·Developmental Biology·I LeivoJ Wartiovaara
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
Aug 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P QinM A Kurpakus
Feb 12, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L M SorokinP Ekblom
Sep 24, 1998·Journal of Theoretical Biology·M R Bryant, P J McDonnell
Jan 26, 1999·Annals of the New York Academy of Sciences·M EkblomP Ekblom
Jan 26, 1999·Annals of the New York Academy of Sciences·N SmythD Edgar
Dec 23, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M FalkP Ekblom
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H Colognato, P D Yurchenco
Jul 13, 2000·Genesis : the Journal of Genetics and Development·I BrownellM Jamrich
Sep 27, 2001·Cell and Tissue Research·A van der Flier, A Sonnenberg
Feb 28, 2003·The Journal of Biological Chemistry·Alex GentleNeville A McBrien
Apr 26, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Peter EkblomJan F Talts
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ronald G GreggBrian A Link
May 27, 2003·Investigative Ophthalmology & Visual Science·Shukti ChakravartiDavid E Birk
Jul 11, 2003·Progress in Retinal and Eye Research·Neville A McBrien, Alex Gentle
Jul 25, 2003·Developments in Ophthalmology·Marita Feldkämper, Frank Schaeffel
Oct 4, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ritva YlikärppäTaina Pihlajaniemi

❮ Previous
Next ❯

Citations

Jan 6, 2010·The Journal of Biological Chemistry·Malia M EdwardsPatsy M Nishina
Feb 15, 2014·TheScientificWorldJournal·Mallika PathaniaMelinda K Duncan
Oct 4, 2016·Cellular and Molecular Life Sciences : CMLS·Yao Yao
Oct 9, 2007·Developmental Biology·Ashley E WebbDavid Kimelman
Aug 2, 2008·Experimental Eye Research·Janice Walker, A Sue Menko
Oct 27, 2009·Developmental Biology·Tania Rozario, Douglas W DeSimone
Sep 21, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Ann C Morris
Feb 24, 2007·Human Molecular Genetics·Douglas B GouldSimon W M John
Aug 4, 2020·Frontiers in Molecular Neuroscience·Lacramioara Fabian, James J Dowling
Apr 20, 2017·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masamoto AoseJohn I Clark
Jan 1, 2021·Matrix Biology : Journal of the International Society for Matrix Biology·JodiRae DeDreuA Sue Menko
May 1, 2021·Cells·Ana Quelle-RegaldieLaura Sánchez
Dec 1, 2006·Seminars in Cell & Developmental Biology·Elizabeth D Wederell, Robb U de Iongh
Jan 2, 2007·Developmental Biology·Peter G FuerstRobert W Burgess
Sep 9, 2008·Experimental Eye Research·Brian P Danysh, Melinda K Duncan

❮ Previous
Next ❯

Datasets Mentioned

BETA
DQ131910

Methods Mentioned

BETA
P25391
PCR
delamination
electrophoresis

Software Mentioned

Vector
Gene
Vector [UNK]
BLAST
[UNK]
BLAST engine

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Tamar SztalThomas E Hall
American Journal of Respiratory Cell and Molecular Biology
R A PierceRobert M Senior
The International Journal of Biochemistry & Cell Biology
Jeffrey H Miner, Bruce L Patton
© 2021 Meta ULC. All rights reserved